Proteomic analyses reveal that Orf virus induces the activation and maturation of mouse bone marrow-derived dendritic cells.

Orf virus (ORFV) is known for its immunostimulatory capacities and has been utilized as an efficient viral vector vaccine in non-permissive host species. Murine bone marrow-derived dendritic cells (BMDCs) are able to react with ORFV. In this study, we aimed to identify pivotal differentially expressed proteins involved in the process of DCs' differentiation in response to ORFV. Our findings showed that ORFV activates the maturation and differentiation of DCs. We further identified and validated seven differentially expressed proteins following ORFV stimulation. With functions in biological processes such as stimulus response, DCs maturation, antigen presentation and Th1 cell activation. Western blot analyses validated the respective changes in protein expression. The huge number of differentially expressed proteins identified in this study will be valuable for elucidating the mechanisms underlying ORFV-induced immunomodulation of murine BMDCs.