Oral and inhalation bioaccessibility of metal(loid)s in chromated copper arsenate (CCA)-contaminated soils: Assessment of particle size influence.

Soil samples adjacent to ten CCA-treated utility poles were collected, sieved into four fractions (<2 mm, 250-90 μm, 90-20 μm and <20 μm), and characterized for their total metal(loid) (As, Cu, Cr, Pb, and Zn) content and physico-chemical properties. Oral bioaccessibility tests were performed using In Vitro Gastrointestinal (IVG) method for fractions 250-90 μm and 90-20 μm. Inhalation bioaccessibility tests were performed in particle size fraction <20 μm using two simulated lung fluids: artificial lysosomal fluid (ALF) and Gamble's solution (GS). The total concentration of metal(loid)s increased with decreasing particle size. Oral As bioaccessibility (%) increased with increasing particle size in 9 out of 10 soils (p < .05), but oral As bioaccessibility expressed in mg/kg was not significantly different for both particle size. Oral Cu bioaccessibility (% and mg/kg) was not influenced by particle size, but oral Cr bioaccessibility (% and mg/kg) increased when reducing particle size (p < .05), although Cr bioaccessibility was very low (< 8%). Oral bioaccessibility (%) of metal(loid)s decreased in the order: Cu > As > Pb > Zn > Cr. Bioaccessibility (%) in simulated lung fluids decreased in the order: Cu > Zn > As > Pb ≈ Cr using ALF, and As > Cu using GS solution. For all elements, inhalation bioaccessibility (% and mg/kg) using ALF was higher than oral bioaccessibility, except for Pb bioaccessibility (mg/kg) in two samples. However, solubility of metal(loid)s in GS presented the lowest values. Copper showed the highest oral and inhalation bioaccessibility (%) and Cr showed the lowest. Moreover, organic matter content and cation exchange capacity in particle size 90-20 μm were negatively correlated with Cu and Pb oral bioaccessibility (%).