Mi Gyeong Jang1,2, Hee Chul Ko2, Se-Jae Kim3,4. 1. Department of Biology, Jeju National University, 102 Jejudaehak-ro, Jeju-si, Jeju Special Self-Governing Province, 63243, Republic of Korea. 2. Biotech Regional Innovation Center, Jeju National University, 102 Jejudaehak-ro, Jeju- si, Jeju Special Self-Governing Province, 63243, Republic of Korea. 3. Department of Biology, Jeju National University, 102 Jejudaehak-ro, Jeju-si, Jeju Special Self-Governing Province, 63243, Republic of Korea. sjkim@jejunu.ac.kr. 4. Biotech Regional Innovation Center, Jeju National University, 102 Jejudaehak-ro, Jeju- si, Jeju Special Self-Governing Province, 63243, Republic of Korea. sjkim@jejunu.ac.kr.
Abstract
BACKGROUND: p-Coumaric acid (p-CA), a phenolic compound abundantly found in edible plants, was found to induce apoptosis in SNU-16 gastric cancer cells. However, the effects of p-CA on the microRNA expression pattern in SNU-16 cells have not been reported. OBJECTIVES: The primary objective of this study was to investigate microRNA expression profiles in p-CA-treated SNU-16 cells. METHODS: SNU-16 cells were treated with 1.5 mM p-CA for 48 h. High-throughput RNA sequencing was performed. The accuracy and validity of the RNA sequencing results were tested by real-time PCR. Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed using the differentially expressed microRNAs (DEMs). RESULTS: Thirty-two DEMs were identified between p-CA-treated and control cells, 9 of which were upregulated, and 23 downregulated. GO and KEGG pathway analyses of the target genes indicated that these genes were involved in a variety of biological functions and several cancer-related pathways. The miRNA interactive network analysis identified hsa-miR-125a-5p, hsa-miR-30a-5p, and hsa-miR-7-5p as the major nodes. CONCLUSIONS: Our findings indicated that p-CA exerted its anticancer effects in SNU-16 gastric cancer cells by modulating the expression of certain microRNAs, providing a better understanding of the anticancer properties of p-CA in gastric cancer cells.
BACKGROUND:p-Coumaric acid (p-CA), a phenolic compound abundantly found in edible plants, was found to induce apoptosis in SNU-16gastric cancer cells. However, the effects of p-CA on the microRNA expression pattern in SNU-16 cells have not been reported. OBJECTIVES: The primary objective of this study was to investigate microRNA expression profiles in p-CA-treated SNU-16 cells. METHODS:SNU-16 cells were treated with 1.5 mM p-CA for 48 h. High-throughput RNA sequencing was performed. The accuracy and validity of the RNA sequencing results were tested by real-time PCR. Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed using the differentially expressed microRNAs (DEMs). RESULTS: Thirty-two DEMs were identified between p-CA-treated and control cells, 9 of which were upregulated, and 23 downregulated. GO and KEGG pathway analyses of the target genes indicated that these genes were involved in a variety of biological functions and several cancer-related pathways. The miRNA interactive network analysis identified hsa-miR-125a-5p, hsa-miR-30a-5p, and hsa-miR-7-5p as the major nodes. CONCLUSIONS: Our findings indicated that p-CA exerted its anticancer effects in SNU-16gastric cancer cells by modulating the expression of certain microRNAs, providing a better understanding of the anticancer properties of p-CA in gastric cancer cells.
Authors: Noha Mokhtar Abd-El-Aziz; Mohamed S Hifnawy; Ahmed A El-Ashmawy; Rehab A Lotfy; Inas Y Younis Journal: Sci Rep Date: 2022-05-25 Impact factor: 4.996