Measured data of Drosophila melanogaster (Diptera Drosophilidae) development and learning and memory behaviour after copper exposition.

This article presents the statistical analysis data from Drosophila melanogaster development (from larvae to adult) and learning and memory retention behavior of a Pavlovian conditioning in male and female flies exposed to copper. While the full data sets are available In the article: Copper decrease associative learning and memory in D. melanogaster, this data-in-brief article includes the detailed statistical analysis performed. Data demonstrates Statistica Software analysis between the subject part of the analysis: 2 treatments x 2 sexes x 2 ages and within subject part of the analysis: 2 treatments x 2 sex x 2 ages x 4 times, repeated measures.

Data description

In this article, we present the detailed statistical analysis performed data from Drosophila melanogaster development (from larvae to adult - Fig. 1) and learning and memory retention behavior (Table 1 and Fig. 2, Fig. 3, Fig. 4) after copper exposition. The full data sets are available on the research article: Copper decrease associative learning and memory in D. melanogaster.

Fig. 1

Statistical analysis of Drosophila melanogaster survival in (A) larvae, (B) pupae, and (C–D) adults after 0.75 mM copper exposition. Data are expressed as mean + −SEM for N = 7 per group (the dependent variables were the number of larvae, pupae or adult male and female flies per each flask; seven independent flasks were analyzed for control and copper-treated groups, flies were exposed to control or Cu2+-containing media from egg to adult life). *p < 0.05; ***p < 0.001 Student T test (compared to control).

Table 1

Raw data within subject part of analysis involving: 2 sex (1 = female and 2 = male), 2 treatments (1 = control and 2 = Cu2+), 2 ages (1 = young and 2 = old) and 4 times (0, 15, 30 and 60 min after shock – dependent variables).

Sex	Treatment	Age	Time 0	Time 15	Time 30	Time 60
1 = female	1 = control	1 = Young
2 = male	2 = Cu2+	2 = old
1	1	1	100	100	60	14,2
1	1	2	83	74	55	17,6
1	2	1	90	84	60	10
1	2	2	80	60	28	11
2	1	1	95	86	66	17,6
2	1	2	80	77	58	10
2	2	1	78	77	55	5,2
2	2	2	72	63	33	5,8
1	1	1	100	100	77	12,5
1	1	2	82	76	52	11
1	2	1	89	82	71	9
1	2	2	82	63	26	5,26
2	1	1	91	83	71	11
2	1	2	78,9	72	53	11
2	2	1	84	74	63	9
2	2	2	80	50	41	6,6
1	1	1	100	97	80	35
1	1	2	75	67	42	0
1	2	1	85	75	50	15
1	2	2	53	43	30	0
2	1	1	90	80	60	20
2	1	2	70	60	45	7
2	2	1	73	67	52	5
2	2	2	60	55	30	5
1	1	1	95	100	70	46
1	1	2	71	64	53	7
1	2	1	77	70	43	12
1	2	2	62	40	32	5
2	1	1	91	87	74	27
2	1	2	71	60	50	0
2	2	1	85	60	57	15
2	2	2	66	50	43	0

Fig. 2

Statistica Software global data of D. melanogaster memory retention test after 0.75 mM copper exposition. Repeated Measures Analysis of Variance with Effects size and Powers demonstrates a significant effect of treatment [F = 54, 258; p < 0.001], age [F = 101, 621; p < 0.001], time [F = 817, 798; p < 0.001], time x treatment [F = 4, 094; p < 0.01] and time x age [F = 6313; p < 0.001].

Fig. 3

Statistica data between subject part of the analysis - 2 treatments (control/Cu2+) x 2 sexes (male/female) x 2 ages (4-days-old/11 days-old). Data demonstrates significant difference between (A) treatments [F (1,24) = 54,258; p < 0.05] and (B) age [F (1,24) = 101,62; p < 0.05], and no significant difference between (C) sex, (D) comparing sex versus treatment, (E) comparing sex versus age and (F) comparing treatment versus age.

Fig. 4

Statistica data within subject part of the analysis - 2 treatment (control/Cu2+) x 2 sex (male/female) x 2 age (4-days-old/11 days-old) x 4 times (O, 15, 30 and 60 min after shock). Repeated measures analysis demonstrates a significant main effect of (A) time [F (3,72) = 817.8; p < 0,0001], (B) time versus treatment [F (3,72) = 4.09; p < 0,01] and (C) time versus age [F (3,72) = 6,31; p < 0,001] interaction. No significant effect comparing (D) time versus sex, (E) time versus sex versus treatment, (F) time versus sex versus age, (G) time versus treatment versus age and (H–K) time versus sex versus treatment versus age at all time points.

Fig. 1 demonstrates the detailed statistical analysis of data evidencing the effect of copper (Cu2+) on D. melanogaster survival in larvae (A – unpaired T test), pupae (B – unpaired T test), total adults and adults divided in male and female (C and D – Two-way ANOVA followed by Newman-Keuls multiple comparisons test). Media ±SEM, R square and F test to compare variances are also demonstrated. Seven independent flasks were analyzed for control and copper-treated groups, the dependent variables were the number of larvae, pupae or adult male and female flies per each flask. Flies were exposed to control or Cu2+-containing media from egg to adult life. GraphPad Prism 6 was used to construct the figures.

This article also demonstrates Statistica data of the effect of Cu2+ on D. melanogaster memory retention test. Table 1 presents the Data within subject part of the analysis: 2 treatments (control/Cu2+) x 2 sex (male/female) x 2 ages (4-days-old/11 days-old) x 4 times (O/15/30/60 min after shock) included in Statistica Software. Repeated Measures Analysis of Variance with Effect Size and Powers was performed using Statistica Software and is illustrated in Fig. 2. Data demonstrates a significant effect of treatment [F = 54, 258; p < 0.001], age [F = 101, 621; p < 0.001], time [F = 817, 798; p < 0.001], time x treatment [F = 4, 094; p < 0.01] and time x age [F = 6313; p < 0.001].

The current effect between subject part of the analysis - 2 treatments (control/Cu2+) x 2 sexes (male/female) x 2 ages (4-days-old/11 days-old) are demonstrated in Fig. 3. It can be observed in Fig. 3 (A) a significant difference between treatments (p < 0.05), (B) significant difference between age (p < 0.05), (C) no significant difference between sex, (D) no significant difference comparing sex versus treatment, (E) no significant difference comparing sex versus age and (F) no significant difference comparing treatment versus age.

The Statistica data within subject part of the analysis: 2 treatments (control/Cu2+) x 2 sex (male/female) x 2 ages (4-days-old/11 days-old) x 4 times (O/15/30/60 min after shock – repeated measure), are demonstrated in Fig. 4. Fig. 4A demonstrates a significant main effect of time [F (3,72) = 817.8; p < 0,0001]. Within subject analysis Fig. 4B indicated a significant difference in time versus treatment [F (3,72) = 4.09; p < 0,01] and Fig. 4. C in time versus age [F (3,72) = 6,31; p < 0,001] interaction. No significant effect comparing time versus sex (Fig. 4D), time versus sex versus treatment (Fig. 4E), time versus sex versus age (Fig. 4F), time versus treatment versus age (Fig. 4G) and time versus sex versus treatment versus age at all time points (Fig. 4 H–K).

Experimental design, materials, and methods

D. melanogaster (Harwich strain) was obtained from the National Species Stock Center (Bowling Green, OH, USA) and maintained following previously described [1] at constant temperature (22-24 °C) and relative humidity (60–70%) under 12-h dark/light cycle conditions. The offspring of approximately 70 flies (5 couples per each flask, in a total of 14 flasks divided into 2 groups) mated during 24 h were used in the present investigation. The flies were exposed to 0 or 0.75 mM Cu2+ during the entire life cycle (from egg to adult age).

Data from official sources were manually acquired. The survival rates of larvae (5 days of exposure), pupae (9 days of exposure) and male and female adult (13 days of exposure) to Cu2+ exposure were visually evaluated. Memory retention test was performed with protocols previously described [2,3].

Graphs were plotted using GraphPad Prism 6 Software and statistical analysis was performed using Statistica Software. Survival significance was assessed by Unpaired T test of student and two-way analysis of variance (ANOVA), followed by Newman-Keuls's post hoc test. Memory retention significance was assessed by a 2 (control/Cu2+) x 2 (male/female) x 2 (4- or 11-days-old) x 4 (trials, immediately, 15, 30 or 60 min after the shock) ANOVA (with trials treated as repeated measure factor). For clarity, results from the between- and within-subject effects (repeated measures) were discussed separately. Differences were considered as statistically significant among groups when p < 0.05.

Specifications table

Subject	Toxicology
Specific subject area	Toxicology of metals in an in vivo model
Type of data	Table, Graphs and Images
How data were acquired.	Data were acquired manually/visuallyModel: Drosophila melanogasterInstruments: Microscope; Apparatus for conditioning and electric shockPrograms: GraphicPad Prism 6 Software and Statistica Software
Data format	Raw, Analyzed and Filtered
Parameters for data collection	Data were collected at constant temperature (22-24 °C) and relative humidity (60–70%). For memory retention test, homogeneous light at all environments and absence of other significant odors that could unfeasible the behavior.
Description of data collection	Survival and Retention memory data were collected visually.Graphs and statistical analysis were constructed using GraphPad Prism 6 Software and Statistica Software.
Data source location	Universidade Federal de Santa MariaSanta Maria/Rio Grande do SulBrazil
Data accessibility	With the article
Related research article	Daniele C. Zamberlan, Paula T. Halmenschelager, Luis F. O. Silva, João B. T. da Rocha. Copper decrease associative learning and memory in Drosophila melanogaster. Science of the total environment. 2019.

Value of the Data• These data testify and reinforce with the insights of its related research paper: Copper decrease associative learning and memory in Drosophila melanogaster • These data serve as a starting point for the scientific community that investigates the potential risks of metals contamination by different sources, like the use of metal-enriched chemicals, fertilizers, organic amendments, and others • The detailed statically analysis described in this article can be used to compare different data, and more surely detect significant results.