Literature DB >> 32459225

Teaching old indicators even more tricks: binding affinity measurements with the guest-displacement assay (GDA).

Stephan Sinn1, Joana Krämer1, Frank Biedermann1.   

Abstract

A simple change has important consequences: the guest-displacement assay (GDA) is introduced which allows for binding affinity determinations of supramolecular complexes with spectroscopically silent hosts and guests. GDA is complementary to indicator-displacement assay for affinity measurements with soluble components, but is superior for insoluble or for weakly binding guests.

Year:  2020        PMID： 32459225     DOI： 10.1039/d0cc01841d

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  5 in total

Review 1.  Molecular Probes, Chemosensors, and Nanosensors for Optical Detection of Biorelevant Molecules and Ions in Aqueous Media and Biofluids.

Authors:  Joana Krämer; Rui Kang; Laura M Grimm; Luisa De Cola; Pierre Picchetti; Frank Biedermann
Journal:  Chem Rev       Date:  2022-01-07       Impact factor: 60.622

2.  Supramolecular capture of highly polar amidosquaraine dye in water with nanomolar affinity and large turn-on fluorescence.

Authors:  Madushani Dharmarwardana; Janel M Dempsey; Sasha Padilla-Coley; Tia S Jarvis; Kejia Shi; Kirk M Atkinson; Bradley D Smith
Journal:  Chem Commun (Camb)       Date:  2021-12-14       Impact factor: 6.222

3.  Benzimidazole-Piperazine-Coumarin/Cucurbit[7]uril Supramolecular Photoinduced Electron Transfer Fluorochromes for Detection of Carnosol by Stimuli-Responsive Dye Displacement and pK a Tuning.

Authors:  Rukayat S Bojesomo; Khaleel I Assaf; Haythem A Saadeh; Lamia A Siddig; Na'il Saleh
Journal:  ACS Omega       Date:  2022-01-05

4.  The Role of Packing, Dispersion, Electrostatics, and Solvation in High-Affinity Complexes of Cucurbit[n]urils with Uncharged Polar Guests.

Authors:  Laura M Grimm; Sebastian Spicher; Boryslav Tkachenko; Peter R Schreiner; Stefan Grimme; Frank Biedermann
Journal:  Chemistry       Date:  2022-05-25       Impact factor: 5.020

5.  Covalent cucurbit[7]uril-dye conjugates for sensing in aqueous saline media and biofluids.

Authors:  Changming Hu; Laura Grimm; Amrutha Prabodh; Ananya Baksi; Alicja Siennicka; Pavel A Levkin; Manfred M Kappes; Frank Biedermann
Journal:  Chem Sci       Date:  2020-09-22       Impact factor: 9.825
  5 in total

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