| Literature DB >> 3245572 |
Y Nagashima1, S Nishio, T Noguchi, O Arakawa, S Kanoh, K Hashimoto.
Abstract
A new method for detection of tetrodotoxin (TTX) by thin-layer chromatography/fast atom bombardment (FAB) mass spectrometry was developed. TTX and/or related substances were separated by TLC on LHP-K high-performance precoated plates, with a solvent system of pyridine:ethyl acetate:acetic acid:water (15:5:3:4). The plates were subjected to positive FAB mass spectrometry, under scanning within a mass range from m/z 100 to 500. TTX was identified by selected ion-monitored chromatograms at m/z 320 (M + H)+ and 302 (M + H - H2O)+, along with full scan positive ion FAB mass spectrometry. The limit of detection for TTX was about 0.1 micrograms. TTX was also detected by cellulose acetate membrane electrophoresis/FAB mass spectrometry.Entities:
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Year: 1988 PMID： 3245572 DOI： 10.1016/0003-2697(88)90386-7
Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365