Naznoosh Shomali1, Okan Onar1, Tuğçe Alkan1, Nergiz Demirtaş2, Ilgaz Akata1, Özlem Yildirim1.
Abstract
OBJECTIVES: Ethanolic extracts of the mushroom species Ganoderma adspersum, Inonotus hispidus, Russula chloroides, and Sarcodon imbricatus were investigated for their polyphenolic contents and biological activities.
MATERIALS AND METHODS: The radical scavenging activity of the extracts was evaluated by 2,2-diphenyl-1-(2,4,6-trinitrophenyl) (DPPH) method and their polyphenolic compounds were determined by high performance liquid chromatography (HPLC) analysis. Furthermore, the activity effects of mushroom extracts on the enzyme glutathione-S-transferase (GST) were also examined. Additionally, the antimicrobial activity of mushroom extracts was evaluated by disc diffusion method.
RESULTS: Ethanolic extract of I. hispidus demonstrated the highest total phenolic content and total flavonoid contents, with 227.23±4.96 mg gallic acid equivalent/g and 42.14±0.20 quercetin equivalent/g, respectively. The highest DPPH radical scavenging activity was observed for ethanolic extracts of I. hispidus, with 10.687±1.643 µg/mL IC50. HPLC analysis demonstrated that R. chloroides was composed of ferulic acid, gallic acid, and myricetin compounds. The highest GST enzyme activity effect was detected with the ethanol extracts of I. hispidus and S. imbricatus. None of the mushroom extracts demonstrated significant inhibition of the bacterial strains used.
CONCLUSION: These results indicate that I. hispidus may be proposed as a new potential source of natural medicine and its potential may be related to its polyphenolic content, which needs further investigation. ©Copyright 2019 Turk J Pharm Sci, Published by Galenos Publishing House.
OBJECTIVES: Ethanolic extracts of the mushroom species Ganoderma adspersum, Inonotus hispidus, Russula chloroides, and Sarcodon imbricatus were investigated for their polyphenolic contents and biological activities.
MATERIALS AND METHODS: The radical scavenging activity of the extracts was evaluated by 2,2-diphenyl-1-(2,4,6-trinitrophenyl) (DPPH) method and their polyphenolic compounds were determined by high performance liquid chromatography (HPLC) analysis. Furthermore, the activity effects of mushroom extracts on the enzyme glutathione-S-transferase (GST) were also examined. Additionally, the antimicrobial activity of mushroom extracts was evaluated by disc diffusion method.
RESULTS: Ethanolic extract of I. hispidus demonstrated the highest total phenolic content and total flavonoid contents, with 227.23±4.96 mg gallic acid equivalent/g and 42.14±0.20 quercetin equivalent/g, respectively. The highest DPPH radical scavenging activity was observed for ethanolic extracts of I. hispidus, with 10.687±1.643 µg/mL IC50. HPLC analysis demonstrated that R. chloroides was composed of ferulic acid, gallic acid, and myricetin compounds. The highest GST enzyme activity effect was detected with the ethanol extracts of I. hispidus and S. imbricatus. None of the mushroom extracts demonstrated significant inhibition of the bacterial strains used.
CONCLUSION: These results indicate that I. hispidus may be proposed as a new potential source of natural medicine and its potential may be related to its polyphenolic content, which needs further investigation. ©Copyright 2019 Turk J Pharm Sci, Published by Galenos Publishing House.
Entities:
Keywords:
Wild mushrooms; antioxidant; detoxification properties; glutathione-S-transferase; polyphenolic compounds
Year: 2019
PMID： 32454708 PMCID： PMC7227972 DOI： 10.4274/tjps.galenos.2018.03274
Source DB: PubMed Journal: Turk J Pharm Sci ISSN： 1304-530X