Ozadheoghene Eriarie Afieroho 1 , Xavier Siwe Noundou 2 , Chiazor P Onyia 1 , Osamuyi H Festus 1 , Elizabeth C Chukwu 1 , Olutayo M Adedokun 3 , Michelle Isaacs 4 , Heinrich C Hoppe 4 , Rui Wm Krause 2 , Kio A Abo 1 Show Affiliations »
Abstract
OBJECTIVES: Several mushrooms species have been reported to be nematophagous and antiprotozoan. This study reported the antiplasmodial and cytotoxic properties of the n-hexane extract from the edible mushroom Pleurotus ostreatus and the isolation of a sterol from the extract. MATERIALS AND METHODS: Antiplasmodial and cytotoxicity assays were done in vitro using the plasmodium lactate dehydrogenase assay and human HeLa cervical cell lines, respectively. The structure of the isolated compound from the n-hexane extract was elucidated using spectroscopic techniques. RESULTS: The n-hexane extract (yield: 0.93% w/w) showed dose dependent antiplasmodial activity with the trend in parasite inhibition of: chloroquine (IC50=0.016 μg/mL) > n-hexane extract (IC50=25.18 μg/mL). It also showed mild cytotoxicity (IC50>100 μg/mL; selectivity index >4) compared to the reference drug emetine (IC50=0.013 μg/mL). The known sterol, ergostan-5,7,22-trien-3-ol, was isolated and characterized from the extract. CONCLUSION: This study reporting for the first time the antiplasmodial activity of P. ostreatus revealed its nutraceutical potential in the management of malaria. ©Copyright 2019 Turk J Pharm Sci, Published by Galenos Publishing House.
OBJECTIVES: Several mushrooms species have been reported to be nematophagous and antiprotozoan. This study reported the antiplasmodial and cytotoxic properties of the n-hexane extract from the edible mushroom Pleurotus ostreatus and the isolation of a sterol from the extract. MATERIALS AND METHODS: Antiplasmodial and cytotoxicity assays were done in vitro using the plasmodium lactate dehydrogenase assay and human HeLa cervical cell lines, respectively. The structure of the isolated compound from the n-hexane extract was elucidated using spectroscopic techniques. RESULTS: The n-hexane extract (yield: 0.93% w/w) showed dose dependent antiplasmodial activity with the trend in parasite inhibition of: chloroquine (IC50=0.016 μg/mL) > n-hexane extract (IC50=25.18 μg/mL). It also showed mild cytotoxicity (IC50>100 μg/mL; selectivity index >4) compared to the reference drug emetine (IC50=0.013 μg/mL). The known sterol, ergostan-5,7,22-trien-3-ol, was isolated and characterized from the extract. CONCLUSION: This study reporting for the first time the antiplasmodial activity of P. ostreatus revealed its nutraceutical potential in the management of malaria. ©Copyright 2019 Turk J Pharm Sci, Published by Galenos Publishing House.
Entities: Chemical
Keywords:
Pleurotus ostreatus; cytotoxicity; ergosterol; malaria; nutraceuticals
Year: 2018
PMID： 32454693 PMCID： PMC7227985 DOI： 10.4274/tjps.18894
Source DB: PubMed Journal: Turk J Pharm Sci ISSN： 1304-530X