Zehra Sarigöl-Kiliç1, Ülkü Ündeğer-Bucurgat1.
Abstract
OBJECTIVES: Pendimethalin and trifluralin are commonly used in many countries to control broadleaf weeds and grassy weed species because of their inhibitor effects on growth and cell division. In this study, we examined the apoptotic and anti-apoptotic potentials of pendimethalin and trifluralin on A549 human non-small lung cancer cells with several concentrations in vitro.
MATERIALS AND METHODS: The expression levels of apoptosis-related genes BCL-2, BAX, CAS3, CAS9, P53, BIRC, and PPIA were examined using quantitative RT-PCR after 24 h treatment of 1, 10, 50, 100 and 500 μM pendimethalin and trifluralin.
RESULTS: The effects of pendimethalin were found more repressive than trifluralin on all studied concentrations. Twenty-four hours' exposure with 100 μM pendimethalin and trifluralin altered the gene expressions, suppressing apoptosis and allowing cancer cells to grow and proliferate.
CONCLUSION: Care should be taken not to exceed the permissible values and residue limits in food during pendimethalin and trifluralin use in order to reduce the possible carcinogenic effects on humans. ©Copyright 2018 Turk J Pharm Sci, Published by Galenos Publishing House.
OBJECTIVES: Pendimethalin and trifluralin are commonly used in many countries to control broadleaf weeds and grassy weed species because of their inhibitor effects on growth and cell division. In this study, we examined the apoptotic and anti-apoptotic potentials of pendimethalin and trifluralin on A549 human non-small lung cancer cells with several concentrations in vitro.
MATERIALS AND METHODS: The expression levels of apoptosis-related genes BCL-2, BAX, CAS3, CAS9, P53, BIRC, and PPIA were examined using quantitative RT-PCR after 24 h treatment of 1, 10, 50, 100 and 500 μM pendimethalin and trifluralin.
RESULTS: The effects of pendimethalin were found more repressive than trifluralin on all studied concentrations. Twenty-four hours' exposure with 100 μM pendimethalin and trifluralin altered the gene expressions, suppressing apoptosis and allowing cancer cells to grow and proliferate.
CONCLUSION: Care should be taken not to exceed the permissible values and residue limits in food during pendimethalin and trifluralin use in order to reduce the possible carcinogenic effects on humans. ©Copyright 2018 Turk J Pharm Sci, Published by Galenos Publishing House.
Entities:
Keywords:
A549; Pendimethalin; apoptosis; gene expressions; trifluralin
Year: 2018
PMID： 32454683 PMCID： PMC7227839 DOI： 10.4274/tjps.94695
Source DB: PubMed Journal: Turk J Pharm Sci ISSN： 1304-530X