Literature DB >> 3244804

Construction of an extrachromosomally replicating transformation vector for Dictyostelium discoideum.

B Leiting1, A Noegel.   

Abstract

An extrachromosomally replicating transformation vector for Dictyostelium discoideum has been constructed using sequences of the endogenous Dictyostelium plasmid Ddp2. This transformation vector pnDeI (9.6 kb) replicates as a high copy number plasmid in Dictyostelium and is located in the nucleus. It has been constructed as shuttle vector containing the Escherichia coli vector pUC19 for replication and selection in E. coli and a part of the Tn903 transposon which confers resistance to G418 for selection in Dictyostelium. In order to show that the vector can be used for cloning and stable propagation of Dictyostelium DNA, a fragment of the Dictyostelium alpha-actinin gene that was marked with a synthetic oligonucleotide was cloned into pnDeI and found to be stably maintained in the extrachromosomal vector without undergoing noticeable recombination with the endogenous gene.

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Year:  1988        PMID： 3244804     DOI： 10.1016/0147-619x(88)90030-3

Source DB:  PubMed          Journal:  Plasmid        ISSN： 0147-619X            Impact factor:   3.466


  8 in total

1.  Site-directed mutations of Dictyostelium actin: disruption of a negative charge cluster at the N terminus.

Authors:  K Sutoh; M Ando; K Sutoh; Y Y Toyoshima
Journal:  Proc Natl Acad Sci U S A       Date:  1991-09-01       Impact factor: 11.205

2.  The hybrid histidine kinase DokA is part of the osmotic response system of Dictyostelium.

Authors:  S C Schuster; A A Noegel; F Oehme; G Gerisch; M I Simon
Journal:  EMBO J       Date:  1996-08-01       Impact factor: 11.598

3.  The extrachromosomal replication of Dictyostelium plasmid Ddp2 requires a cis-acting element and a plasmid-encoded trans-acting factor.

Authors:  B Leiting; I J Lindner; A A Noegel
Journal:  Mol Cell Biol       Date:  1990-07       Impact factor: 4.272

4.  Mutagenesis and gene identification in Dictyostelium by shotgun antisense.

Authors:  T P Spann; D A Brock; D F Lindsey; S A Wood; R H Gomer
Journal:  Proc Natl Acad Sci U S A       Date:  1996-05-14       Impact factor: 11.205

5.  Transcript and sequence analysis of a 5.1 kb contiguous fragment of Dictyostelium discoideum plasmid Ddp1 that contains the origin of replication and codes for several transcripts.

Authors:  C B Gurniak; A G Bang; A A Noegel
Journal:  Curr Genet       Date:  1990-04       Impact factor: 3.886

6.  Amino acid substitutions in the Dictyostelium G alpha subunit G alpha 2 produce dominant negative phenotypes and inhibit the activation of adenylyl cyclase, guanylyl cyclase, and phospholipase C.

Authors:  K Okaichi; A B Cubitt; G S Pitt; R A Firtel
Journal:  Mol Biol Cell       Date:  1992-07       Impact factor: 4.138

7.  A negative transcriptional control region of a developmentally-regulated gene co-localizes with the origin of replication of an endogenous plasmid in Dictyostelium.

Authors:  J A Powell; J Galindo; R A Firtel
Journal:  Nucleic Acids Res       Date:  1992-06-11       Impact factor: 16.971

8.  Expression vectors for studying cytoskeletal proteins in Dictyostelium discoideum.

Authors:  Menno L W Knetsch; Georgios Tsiavaliaris; Sabine Zimmermann; Ursula Rühl; Dietmar J Manstein
Journal:  J Muscle Res Cell Motil       Date:  2002       Impact factor: 2.698
  8 in total

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