Dong Liu1, Yanzhen Xu1, Jinxing Feng2, Jialin Yu3, Jinjie Huang1, Zhiguang Li1. 1. Department of Neonatology, People's Hospital of Shenzhen, 2nd Clinical Medical College of Jinan University, Shenzhen, People's Republic of China. 2. Department of Neonatology, Shenzhen Children's Hospital, Shenzhen, People's Republic of China. 3. Department of Pediatrics, Shenzhen University General Hospital, Shenzhen, People's Republic of China.
Abstract
OBJECTIVE: This study investigates the expression levels of mucin 1 (MUC1), MUC2, occludin, and zonula occludens-1 (ZO-1) in necrotizing enterocolitis (NEC). STUDY DESIGN: Intestinal specimens of surgical patients suffering from NEC (the NEC group) and intestinal specimens of patients with congenital intestinal atresia (the control group) were collected. Immunohistochemical changes in MUC1, MUC2, occludin, and ZO-1 were compared between the two groups. RESULTS: Our study showed a significant decrease in the expression levels of MUC1 (p = 0.004), MUC2 (p = 0.001), occludin (p = 0.004), and ZO-1 (p = 0.013) in neonates suffering from NEC as compared with the control group. CONCLUSION: Mucins and tight junctions are severely altered in NEC neonates. This finding might provide clues for rupture of the intestinal barrier. Further research is needed to investigate the gene expression as well as the exact mechanisms behind these changes. This will help us better understand the role of the intestinal barrier in NEC. KEY POINTS: · Mucins and tight junctions are severely altered in NEC neonates.. · We first demonstrate that the expression levels of MUC1are obviously reduced in neonates suffering from NEC.. · Expression levels of MUC2, occludin, and ZO-1, are also significantly decreased in neonates suffering from NEC.. Thieme. All rights reserved.
OBJECTIVE: This study investigates the expression levels of mucin 1 (MUC1), MUC2, occludin, and zonula occludens-1 (ZO-1) in necrotizing enterocolitis (NEC). STUDY DESIGN: Intestinal specimens of surgical patients suffering from NEC (the NEC group) and intestinal specimens of patients with congenital intestinal atresia (the control group) were collected. Immunohistochemical changes in MUC1, MUC2, occludin, and ZO-1 were compared between the two groups. RESULTS: Our study showed a significant decrease in the expression levels of MUC1 (p = 0.004), MUC2 (p = 0.001), occludin (p = 0.004), and ZO-1 (p = 0.013) in neonates suffering from NEC as compared with the control group. CONCLUSION: Mucins and tight junctions are severely altered in NEC neonates. This finding might provide clues for rupture of the intestinal barrier. Further research is needed to investigate the gene expression as well as the exact mechanisms behind these changes. This will help us better understand the role of the intestinal barrier in NEC. KEY POINTS: · Mucins and tight junctions are severely altered in NEC neonates.. · We first demonstrate that the expression levels of MUC1are obviously reduced in neonates suffering from NEC.. · Expression levels of MUC2, occludin, and ZO-1, are also significantly decreased in neonates suffering from NEC.. Thieme. All rights reserved.
Authors: N Deianova; S El Manouni El Hassani; E A Struijs; E E W Jansen; A Bakkali; M A van de Wiel; W P de Boode; C V Hulzebos; A H van Kaam; B W Kramer; E d'Haens; D C Vijlbrief; M M van Weissenbruch; W J de Jonge; M A Benninga; H J Niemarkt; N K H de Boer; T G J de Meij Journal: Sci Rep Date: 2022-07-19 Impact factor: 4.996
Authors: Hala Chaaban; Maulin M Patel; Kathryn Burge; Jeffrey V Eckert; Cristina Lupu; Ravi S Keshari; Robert Silasi; Girija Regmi; MaJoi Trammell; David Dyer; Steven J McElroy; Florea Lupu Journal: Microorganisms Date: 2022-02-27