| Literature DB >> 32446238 |
Francine de Souza Sossella1, Alan Rempel1, Janayna Monroe Araújo Nunes2, Gabriele Biolchi3, Regina Migliavaca4, Ana Carolina Farezin Antunes3, Jorge Alberto Vieira Costa2, Marcelo Hemkemeier2, Luciane Maria Colla5.
Abstract
This study aimed to assess the harvesting of Spirulina platensis using coagulants and electrocoagulation-flotation (ECF) and to evaluate its influence on enzymatic hydrolysis. Using nine chemical coagulants, we obtained a biomass harvesting efficiency of up to 99.5%. Using ECF, the harvesting efficiency at the aluminum and carbon electrode was 98%-99% and 33.8%-86.9%, respectively. Hydrolysis efficiency (HE) with amylases varied from 17% to 42%, and the degree of hydrolysis (DH) with proteases varied from 1.26% to 4.07%, compared with an HE of 31% and a DH of 3.57% in the centrifuged biomass. Compared to an HE of 61.75% for the centrifuged biomass, and HE of 99% and 85.46% was obtained for the biomass harvested using the aluminum and carbon electrodes. The HEs with the electrodes were better than those with the alternative methods and centrifugation; hence, with some optimization, the biomass harvested could be used for enzymatic hydrolysis.Entities:
Keywords: Amylases; Microalgae; Physicochemical separation; Proteases; Saccharification
Year: 2020 PMID: 32446238 DOI: 10.1016/j.biortech.2020.123526
Source DB: PubMed Journal: Bioresour Technol ISSN: 0960-8524 Impact factor: 9.642