The microorganism and biochar-augmented bioreactive top-layer soil for degradation removal of 2,4-dichlorophenol from surface runoff.

Surface runoff is one of the major pollution sources impacting the quality of the surrounding waterbody. In this study, a highly-bioreactive top-layer soil incorporated with microorganism (BO) and peanut shell (PS) biochar or dairy manure (DM) biochar was proposed for removal of 2,4-dichlorophenol (2,4-DCP) from contaminated surface runoff. Both batch test and sandbox experiment consistently revealed that PS coupled with BO amendment (PS + BO) was most effective for sorption and degradation of 2,4-DCP, compared to BO and DM alone or in combination. About 77% of 6000 μg∙L-1 2,4-DCP was absorbed within 36 h in the original low permeability bioreactive PS + BO soil layer (15 cm long×15 cm wide×4.5 cm deep) with the 0.33 L∙day-1 processing capacity of surface runoff. Increasing the addition of quartz sand into the bioreactor soil layer by threefold the original bioreactor improved the processing capacity to 17.5 L∙day-1. However, this permeability-optimized bioreactive layer was still not large enough to remove 2,4-DCP completely. The optimized scale by the multi-process coupling model of the convection, dispersion, adsorption, and degradation was 60 cm long × 60 cm wide × 18 cm deep where the processing capacity of 280 L·day-1reached and 97.3% of 2,4-DCP was removed, correspondingly the 2,4-DCP concentration could meet the standard limit. In addition, the obtained model parameters showed that the biochar or microorganism significantly decreased the dispersion coefficient D of 2,4-DCP in the bioreactive layer. The 2,4-DCP distribution coefficient Kd, and first-order reaction rate λ in the PS+BO system significantly greater than that in the control, BO, and PS systems. Results from this study indicated that the top-layer soil incorporated with microorganisms and biochar is a feasible and effective approach for the surface runoff treatment.