OBJECTIVES: Psoriasis is an immunodeficient skin disorder, and its exact pathogenesis is unclear. Monozygotic twins are presumed to be genetically identical, and their phenotypic differences may be due to transcriptional regulation or epigenome factors. To explain the inconsistency between twins, we have collected 3 pairs of monozygotic twins who are discordant for psoriasis. METHODS: Reduced representation of bisulfite sequencing and RNA sequencing was conducted using the peripheral blood of the twins to find the genes playing important roles in psoriasis pathogenesis. RESULTS: As a result, we found methylation diversity in four genes (MAST3, MTOR, PM20D1 and ZNF99), and we also found 9 differentially expressed genes (PPAN-P2RY11, PIGV, RPS18, TMEM121, KIF21A, KCNH2, WNT10B, PRX and CDH24) by RNA sequencing. According to the conjoint analysis of methylation and the mRNA results, PTPN6, CCL5, NFATC1 and PRF1 were found to be closely related to psoriasis. We then annotated the genes to explore the associations between these genes and psoriasis. CONCLUSIONS: These findings provide a better understanding of psoriasis that can improve the diagnosis and treatment of the disease.
OBJECTIVES:Psoriasis is an immunodeficient skin disorder, and its exact pathogenesis is unclear. Monozygotic twins are presumed to be genetically identical, and their phenotypic differences may be due to transcriptional regulation or epigenome factors. To explain the inconsistency between twins, we have collected 3 pairs of monozygotic twins who are discordant for psoriasis. METHODS: Reduced representation of bisulfite sequencing and RNA sequencing was conducted using the peripheral blood of the twins to find the genes playing important roles in psoriasis pathogenesis. RESULTS: As a result, we found methylation diversity in four genes (MAST3, MTOR, PM20D1 and ZNF99), and we also found 9 differentially expressed genes (PPAN-P2RY11, PIGV, RPS18, TMEM121, KIF21A, KCNH2, WNT10B, PRX and CDH24) by RNA sequencing. According to the conjoint analysis of methylation and the mRNA results, PTPN6, CCL5, NFATC1 and PRF1 were found to be closely related to psoriasis. We then annotated the genes to explore the associations between these genes and psoriasis. CONCLUSIONS: These findings provide a better understanding of psoriasis that can improve the diagnosis and treatment of the disease.
Authors: Veronica Davalos; Carlos A García-Prieto; Gerardo Ferrer; Sergio Aguilera-Albesa; Juan Valencia-Ramos; Agustí Rodríguez-Palmero; Montserrat Ruiz; Laura Planas-Serra; Iolanda Jordan; Iosune Alegría; Patricia Flores-Pérez; Verónica Cantarín; Victoria Fumadó; Maria Teresa Viadero; Carlos Rodrigo; Maria Méndez-Hernández; Eduardo López-Granados; Roger Colobran; Jacques G Rivière; Pere Soler-Palacín; Aurora Pujol; Manel Esteller Journal: EClinicalMedicine Date: 2022-06-25
Authors: Matteo Vecellio; Elvezia Maria Paraboschi; Angela Ceribelli; Natasa Isailovic; Francesca Motta; Giulia Cardamone; Michela Robusto; Rosanna Asselta; Sonia Brescianini; Francesco Sacrini; Antonio Costanzo; Maria De Santis; Maria Antonietta Stazi; Stefano Duga; Carlo Selmi Journal: Front Cell Dev Biol Date: 2021-11-24