Wen-Ping Guo1, Guo-Qing Chen2, Guang-Cheng Xie3, Luan-Ying Du4, Quan Tang5. 1. Department of Pathogenic Biology, College of Basic Medicine, Chengde Medical University, Chengde, Hebei, China. Electronic address: guowenping@nwsuaf.edu.cn. 2. Yancheng Center for Disease Control and Prevention, Yancheng, Jiangsu, China. Electronic address: 78931451@qq.com. 3. Department of Pathogenic Biology, College of Basic Medicine, Chengde Medical University, Chengde, Hebei, China. Electronic address: xieguangcheng123@126.com. 4. Department of Pathogenic Biology, College of Basic Medicine, Chengde Medical University, Chengde, Hebei, China. Electronic address: luanyingd@126.com. 5. Yancheng Center for Disease Control and Prevention, Yancheng, Jiangsu, China. Electronic address: 1610019733@qq.com.
Abstract
OBJECTIVES: To better understand the spectrums of pathogens causing herpangina and circulation of Coxsackievirus A4 in Yancheng, China. METHODS: Stool samples from herpangina and HFMD cases were collected. Real Time PCR Kits was used to identify Enterovirus 71, CV-A16 and CV-A6, and nested reverse transcription PCR (nRT-PCR) to detect the other enterovirus types. Complete VP1 and genome sequence of CV-A4 were amplified by using nRT-PCR. Genetic, phylogenetic and recombination analysis were performed. RESULTS: Co-circulation of three recombinant CV-A4 groups, including one novel (C2 lineage), was identified in Yancheng, China, 2016 and 2018. One was the major causative agent of herpangina, and another two were responsible for HFMD. Phylogenetic and recombination analysis indicated that the non-structural region of their genome originated from the same ancestry and subsequently adaptation. C2 lineage of CV-A4 group may be introduced from countries outside China and its genome occurred recombination in China. CONCLUSION: Novel recombinant CV-A4 was mainly associated with herpanginain in Yancheng, 2018, China. C2 lineage of CV-A4 group with recombinant non-structural region was also identified in HFMD patients.
OBJECTIVES: To better understand the spectrums of pathogens causing herpangina and circulation of Coxsackievirus A4 in Yancheng, China. METHODS: Stool samples from herpangina and HFMD cases were collected. Real Time PCR Kits was used to identify Enterovirus 71, CV-A16 and CV-A6, and nested reverse transcription PCR (nRT-PCR) to detect the other enterovirus types. Complete VP1 and genome sequence of CV-A4 were amplified by using nRT-PCR. Genetic, phylogenetic and recombination analysis were performed. RESULTS: Co-circulation of three recombinant CV-A4 groups, including one novel (C2 lineage), was identified in Yancheng, China, 2016 and 2018. One was the major causative agent of herpangina, and another two were responsible for HFMD. Phylogenetic and recombination analysis indicated that the non-structural region of their genome originated from the same ancestry and subsequently adaptation. C2 lineage of CV-A4 group may be introduced from countries outside China and its genome occurred recombination in China. CONCLUSION: Novel recombinant CV-A4 was mainly associated with herpanginain in Yancheng, 2018, China. C2 lineage of CV-A4 group with recombinant non-structural region was also identified in HFMD patients.
Authors: Zhicheng Du; Yong Huang; Wayne R Lawrence; Jianxiong Xu; Zhicong Yang; Jianyun Lu; Zhoubin Zhang; Yuantao Hao Journal: Int J Environ Res Public Health Date: 2021-01-02 Impact factor: 3.390