Matthew L Stoll1, L Wayne Duck2, Margaret H Chang3, Robert A Colbert4, Peter A Nigrovic5, Susan D Thompson6, Charles O Elson7. 1. University of Alabama at Birmingham (UAB), Departments of Pediatrics, 1601 4(th) Ave South Suite G10, Birmingham, AL 35233, USA. Electronic address: mstoll@peds.uab.edu. 2. UAB, Department of Medicine. 1825 University Blvd, Shelby 631, Birmingham, AL 35294, USA. Electronic address: Duck@uab.edu. 3. Boston Children's Hospital (BCH), Division of Immunology. Fegan 6, 300 Longwood Avenue, Boston, MA 02115, USA. Electronic address: Margaret.Chang@childrens.harvard.edu. 4. Cincinnati Children's Hospital Medical Center (CCHMC), Center for Autoimmune Genomics and Etiology, University of Cincinnati College of Medicine, Immunology Graduate Program. 3333 Burnet Avenue, Cincinnati, OH 45229, USA. Electronic address: Colbert@mail.nih.gov. 5. BCH, Division of Immunology, Brigham and Women's Hospital, Division of Rheumatology, Inflammation, and Immunity, Hale 6002L, 60 Fenwood Road, Boston, MA, USA 02115.. Electronic address: PNigrovic@BWH.Harvard.edu. 6. CCHMC, Center for Autoimmune Genomics and Etiology, University of Cincinnati College of Medicine, Immunology Graduate Program, 3333 Burnet Avenue, Cincinnati, OH 45229, USA. Electronic address: COElson@UABMC.edu. 7. UAB, Department of Medicine, 1825 University Blvd, Shelby 607, Birmingham, AL 35294, USA. Electronic address: COElson@uabmc.edu.
Abstract
OBJECTIVES: Patients with Crohn's disease often produce antibodies against flagellated intestinal bacteria. There are mixed data as to whether such antibodies are present in patients with spondyloarthritis. Our objectives were to evaluate for the presence of antibodies against intestinal organisms in children with enthesitis related arthritis (ERA). METHODS: Children with ERA and healthy controls were recruited at three sites. Sera were plated on a nitrocellulose array and incubated with labelled antibodies to human IgA and IgG. RESULTS: At UAB, patients and controls had similar antibody levels against the majority of the bacteria selected, with the exception of increased IgA antibodies among ERA patients against Prevotella oralis (1231 [IQR 750, 2566] versus 706 [IQR 428, 1106], p = .007.) These findings were partially validated at a second but not at a third site. CONCLUSIONS: ERA patients may produce increased IgA antibodies against P. oralis. The possible significance of this finding bears further exploration.
OBJECTIVES:Patients with Crohn's disease often produce antibodies against flagellated intestinal bacteria. There are mixed data as to whether such antibodies are present in patients with spondyloarthritis. Our objectives were to evaluate for the presence of antibodies against intestinal organisms in children with enthesitis related arthritis (ERA). METHODS:Children with ERA and healthy controls were recruited at three sites. Sera were plated on a nitrocellulose array and incubated with labelled antibodies to humanIgA and IgG. RESULTS: At UAB, patients and controls had similar antibody levels against the majority of the bacteria selected, with the exception of increased IgA antibodies among ERA patients against Prevotella oralis (1231 [IQR 750, 2566] versus 706 [IQR 428, 1106], p = .007.) These findings were partially validated at a second but not at a third site. CONCLUSIONS: ERA patients may produce increased IgA antibodies against P. oralis. The possible significance of this finding bears further exploration.
Authors: Raul Y Tito; Heleen Cypers; Marie Joossens; Gaëlle Varkas; Liesbet Van Praet; Elien Glorieus; Filip Van den Bosch; Martine De Vos; Jeroen Raes; Dirk Elewaut Journal: Arthritis Rheumatol Date: 2016-12-01 Impact factor: 10.995
Authors: Stephan R Targan; Carol J Landers; Huiying Yang; Michael J Lodes; Yingzi Cong; Konstantinos A Papadakis; Eric Vasiliauskas; Charles O Elson; Robert M Hershberg Journal: Gastroenterology Date: 2005-06 Impact factor: 22.682
Authors: Benjamin S Christmann; Thomas R Abrahamsson; Charles N Bernstein; L Wayne Duck; Peter J Mannon; Göran Berg; Bengt Björkstén; Maria C Jenmalm; Charles O Elson Journal: J Allergy Clin Immunol Date: 2015-05-23 Impact factor: 10.793
Authors: L Wayne Duck; Mark R Walter; Jan Novak; Denise Kelly; Maurizio Tomasi; Yingzi Cong; Charles O Elson Journal: Inflamm Bowel Dis Date: 2007-10 Impact factor: 5.325
Authors: M Breban; J L Fernández-Sueiro; J A Richardson; R R Hadavand; S D Maika; R E Hammer; J D Taurog Journal: J Immunol Date: 1996-01-15 Impact factor: 5.422
Authors: Sabina Górska; Wojciech Jachymek; Jacek Rybka; Magdalena Strus; Piotr B Heczko; Andrzej Gamian Journal: Carbohydr Res Date: 2009-09-27 Impact factor: 2.104
Authors: Matthew L Stoll; Ranjit Kumar; Casey D Morrow; Elliot J Lefkowitz; Xiangqin Cui; Anna Genin; Randy Q Cron; Charles O Elson Journal: Arthritis Res Ther Date: 2014-11-30 Impact factor: 5.156
Authors: Matthew L Stoll; Pamela F Weiss; Jennifer E Weiss; Peter A Nigrovic; Barbara S Edelheit; S Lou Bridges; Maria I Danila; Charles H Spencer; Marilynn G Punaro; Kenneth Schikler; Andreas Reiff; Ranjit Kumar; Randy Q Cron; Casey D Morrow; Elliot J Lefkowitz Journal: Arthritis Res Ther Date: 2018-01-30 Impact factor: 5.156