Dina Sabry1, Samar Marzouk2, Reem Zakaria2, Heba A Ibrahim3, Mai Samir2. 1. Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Giza, Egypt. dinasabdry@kasralainy.edu.eg. 2. Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Giza, Egypt. 3. Pathology Department, Faculty of Medicine, Cairo University, Giza, Egypt.
Abstract
AIM: The aim of the current study was to evaluate the therapeutic and regenerative effects of MSCs derived exosomes in the treatment of type 1 DM and to compare its effects with MSCs themselves. The experiment was done on forty albino rats grouped as follows, group (1): Ten healthy rats, group (2): Ten induced type 1 DM rats, group (3): Ten induced type 1 DM rats received exosomes intraperitoneally, and group (4): Ten induced type 1 DM rats received MSCs intraperitoneally. Serum glucose and plasma insulin levels were assessed weekly. QRT-PCR was done to assess regeneration of pancreatic beta cells by measuring insulin, Pdx1, Smad2, Smad3 and TGFβ genes. Additionally, histopathological and immune-histochemical examinations were done to confirm pancreatic tissue regeneration. RESULTS: Regarding the assessed genes (insulin, Pdx1, Smad2, Smad3 and Tgfβ) gene expression in MSCs treated group showed significant increase compared to diabetic group (p value < 0.001) and gene expression in exosomes treated group was increased significantly compared to diabetic and MSCs treated groups (p value < 0.001). Histopathological and immune-histochemical examination revealed regeneration of pancreatic islets in both treated groups. CONCLUSION: MSCs Derived exosomes showed superior therapeutic and regenerative results than MSCs themselves.
AIM: The aim of the current study was to evaluate the therapeutic and regenerative effects of MSCs derived exosomes in the treatment of type 1 DM and to compare its effects with MSCs themselves. The experiment was done on forty albino rats grouped as follows, group (1): Ten healthy rats, group (2): Ten induced type 1 DMrats, group (3): Ten induced type 1 DMrats received exosomes intraperitoneally, and group (4): Ten induced type 1 DMrats received MSCs intraperitoneally. Serum glucose and plasma insulin levels were assessed weekly. QRT-PCR was done to assess regeneration of pancreatic beta cells by measuring insulin, Pdx1, Smad2, Smad3 and TGFβ genes. Additionally, histopathological and immune-histochemical examinations were done to confirm pancreatic tissue regeneration. RESULTS: Regarding the assessed genes (insulin, Pdx1, Smad2, Smad3 and Tgfβ) gene expression in MSCs treated group showed significant increase compared to diabetic group (p value < 0.001) and gene expression in exosomes treated group was increased significantly compared to diabetic and MSCs treated groups (p value < 0.001). Histopathological and immune-histochemical examination revealed regeneration of pancreatic islets in both treated groups. CONCLUSION: MSCs Derived exosomes showed superior therapeutic and regenerative results than MSCs themselves.
Authors: Jordan Mattke; Srividya Vasu; Carly M Darden; Kenjiro Kumano; Michael C Lawrence; Bashoo Naziruddin Journal: Front Endocrinol (Lausanne) Date: 2021-08-10 Impact factor: 5.555