Literature DB >> 3243033

Inactivation of the alpha-actinin gene in Dictyostelium.

A A Noegel1, W Witke.   

Abstract

alpha-Actinin-negative transformants of Dictyostelium have been obtained by transforming cells with a transformation vector carrying part of the alpha-actinin gene in either sense or antisense orientation. The transformants did not produce detectable alpha-actinin anymore and contained an altered RNA lacking the 3' part of the coding sequences. The deficiency in alpha-actinin was due to an integration of the transformation vector into the gene, since it could be detected by Southern blot analysis in the endogenous gene.

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Year:  1988        PMID： 3243033     DOI： 10.1002/dvg.1020090429

Source DB:  PubMed          Journal:  Dev Genet        ISSN： 0192-253X


  8 in total

1.  Sequence similarity of the amino-terminal domain of Drosophila beta spectrin to alpha actinin and dystrophin.

Authors:  T J Byers; A Husain-Chishti; R R Dubreuil; D Branton; L S Goldstein
Journal:  J Cell Biol       Date:  1989-10       Impact factor: 10.539

2.  Mechanical perturbation elicits a phenotypic difference between Dictyostelium wild-type cells and cytoskeletal mutants.

Authors:  L Eichinger; B Köppel; A A Noegel; M Schleicher; M Schliwa; K Weijer; W Witke; P A Janmey
Journal:  Biophys J       Date:  1996-02       Impact factor: 4.033

3.  Positive selection for Dictyostelium discoideum mutants lacking UMP synthase activity based on resistance to 5-fluoroorotic acid.

Authors:  D Kalpaxis; I Zündorf; H Werner; N Reindl; E Boy-Marcotte; M Jacquet; T Dingermann
Journal:  Mol Gen Genet       Date:  1991-03

4.  Molecular genetics of Drosophila alpha-actinin: mutant alleles disrupt Z disc integrity and muscle insertions.

Authors:  E Fyrberg; M Kelly; E Ball; C Fyrberg; M C Reedy
Journal:  J Cell Biol       Date:  1990-06       Impact factor: 10.539

5.  Actin binding domains direct actin-binding proteins to different cytoskeletal locations.

Authors:  Raymond W Washington; David A Knecht
Journal:  BMC Cell Biol       Date:  2008-02-13       Impact factor: 4.241

6.  A 27,000-D core of the Dictyostelium 34,000-D protein retains Ca(2+)-regulated actin cross-linking but lacks bundling activity.

Authors:  M Fechheimer; R Furukawa
Journal:  J Cell Biol       Date:  1993-03       Impact factor: 10.539

7.  Perturbations of Drosophila alpha-actinin cause muscle paralysis, weakness, and atrophy but do not confer obvious nonmuscle phenotypes.

Authors:  E M Roulier; C Fyrberg; E Fyrberg
Journal:  J Cell Biol       Date:  1992-02       Impact factor: 10.539

8.  The Dictyostelium gelation factor shares a putative actin binding site with alpha-actinins and dystrophin and also has a rod domain containing six 100-residue motifs that appear to have a cross-beta conformation.

Authors:  A A Noegel; S Rapp; F Lottspeich; M Schleicher; M Stewart
Journal:  J Cell Biol       Date:  1989-08       Impact factor: 10.539
  8 in total

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