Literature DB >> 32429933

Correction to: PTH Derivative promotes wound healing via synergistic multicellular stimulating and exosomal activities.

Yi-Fan Shen1, Jing-Huan Huang1, Kai-Yang Wang1, Jin Zheng2, Lin Cai3, Hong Gao4, Xiao-Lin Li5, Jing-Feng Li6.   

Abstract

An amendment to this paper has been published and can be accessed via the original article.

Entities:  

Year:  2020        PMID: 32429933      PMCID: PMC7236313          DOI: 10.1186/s12964-020-00593-y

Source DB:  PubMed          Journal:  Cell Commun Signal        ISSN: 1478-811X            Impact factor:   5.712


Correction to: Cell Commun Signal (2020) 18:40 https://doi.org/10.1186/s12964-020-00541-w Following publication of the original article [1], two mistakes were noticed in Fig. 4 and Fig. 6. The pictures describing the effects of 0.1 nM PTHrP-2 group on migration of HUVEC in Fig. 4 and Control and HFF-1-Exos groups on migration of HFF-1 cells in Fig. 6 are incorrect. The correct figures are supplied below in this correction article. The figure legends were not changed.
Fig. 4

Proliferation of HUVECs (a), HFF-1 cells (b), HaCaTs (c) incubated for 0, 1, 3, or 7 days in conditioned medium with different drug concentrations from days 0 and 6. d Effects of PTHrP-2 on migration of HUVECs, HFF-1 cells and HaCaTs and the tube formation assay of HUVECs. e Quantitation of HUVECs, HFF-1 cells and HaCaTs migration (violet stained cells) using a Transwell chamber. The quantitative evaluation of the number of nodes formed in the culture plate with different drug concentrations after 8 h. f Immunofluorescence images of HUVECs and HFF-1 incubated in each group on day 3. Cytoskeleton and cell nuclei are stained red and blue, VEGF and Collagen I are stained green in the picture taken by the laser scanning confocal microscopy. g VEGF and Collagen I secretion by HUVEC and HFF-1 incubated for 3 days in media with different drug concentrations. h Akt and Erk1/2 phosphorylation level in HUVEC and HFF-1 treated with different drug concentrations

Fig. 6

a TEM images of HFF-1-Exos. b Exosome surface markers detected by Western blotting (Alix, Tsg101, CD9). The experiment was repeated three times in order to confirm the stability of the phenomena. c Size distribution of exosomes. Particle concentration, particle size and video frame of exosomes were analyzed by FNA (d) and NTA (e). Total protein levels (f) in HFF-1-Exos and PTHrP-2-HFF-1-Exos. g The uptake of exosomes by HUVECs and HFF-1 cells. Cytoskeleton, exosomes and cell nuclei are stained green, red and blue in the picture taken by the laser scanning confocal microscopy. h Proliferation of HUVECs and HFF-1 cells incubated for 0, 1, 3, or 7 days in conditioned medium with HFF-1-Exos and PTHrP-2-HFF-1-Exos from days 0 and 6. I Effects of HFF-1-Exos on migration of HUVECs and HFF-1 cells and the tube formation assay of HUVECs. j Quantitation of HUVECs and HFF-1 cells migration (violet stained cells) using a Transwell chamber. The quantitative evaluation of the number of nodes formed in the culture plate with different conditions of culture after 8 h

Proliferation of HUVECs (a), HFF-1 cells (b), HaCaTs (c) incubated for 0, 1, 3, or 7 days in conditioned medium with different drug concentrations from days 0 and 6. d Effects of PTHrP-2 on migration of HUVECs, HFF-1 cells and HaCaTs and the tube formation assay of HUVECs. e Quantitation of HUVECs, HFF-1 cells and HaCaTs migration (violet stained cells) using a Transwell chamber. The quantitative evaluation of the number of nodes formed in the culture plate with different drug concentrations after 8 h. f Immunofluorescence images of HUVECs and HFF-1 incubated in each group on day 3. Cytoskeleton and cell nuclei are stained red and blue, VEGF and Collagen I are stained green in the picture taken by the laser scanning confocal microscopy. g VEGF and Collagen I secretion by HUVEC and HFF-1 incubated for 3 days in media with different drug concentrations. h Akt and Erk1/2 phosphorylation level in HUVEC and HFF-1 treated with different drug concentrations a TEM images of HFF-1-Exos. b Exosome surface markers detected by Western blotting (Alix, Tsg101, CD9). The experiment was repeated three times in order to confirm the stability of the phenomena. c Size distribution of exosomes. Particle concentration, particle size and video frame of exosomes were analyzed by FNA (d) and NTA (e). Total protein levels (f) in HFF-1-Exos and PTHrP-2-HFF-1-Exos. g The uptake of exosomes by HUVECs and HFF-1 cells. Cytoskeleton, exosomes and cell nuclei are stained green, red and blue in the picture taken by the laser scanning confocal microscopy. h Proliferation of HUVECs and HFF-1 cells incubated for 0, 1, 3, or 7 days in conditioned medium with HFF-1-Exos and PTHrP-2-HFF-1-Exos from days 0 and 6. I Effects of HFF-1-Exos on migration of HUVECs and HFF-1 cells and the tube formation assay of HUVECs. j Quantitation of HUVECs and HFF-1 cells migration (violet stained cells) using a Transwell chamber. The quantitative evaluation of the number of nodes formed in the culture plate with different conditions of culture after 8 h The authors sincerely apologize for having this unintentional error in the article, and apologize for any inconvenience caused.
  1 in total

1.  PTH Derivative promotes wound healing via synergistic multicellular stimulating and exosomal activities.

Authors:  Yi-Fan Shen; Jing-Huan Huang; Kai-Yang Wang; Jin Zheng; Lin Cai; Hong Gao; Xiao-Lin Li; Jing-Feng Li
Journal:  Cell Commun Signal       Date:  2020-03-09       Impact factor: 5.712

  1 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.