| Literature DB >> 32429933 |
Yi-Fan Shen1, Jing-Huan Huang1, Kai-Yang Wang1, Jin Zheng2, Lin Cai3, Hong Gao4, Xiao-Lin Li5, Jing-Feng Li6.
Abstract
An amendment to this paper has been published and can be accessed via the original article.Entities:
Year: 2020 PMID: 32429933 PMCID: PMC7236313 DOI: 10.1186/s12964-020-00593-y
Source DB: PubMed Journal: Cell Commun Signal ISSN: 1478-811X Impact factor: 5.712
Fig. 4Proliferation of HUVECs (a), HFF-1 cells (b), HaCaTs (c) incubated for 0, 1, 3, or 7 days in conditioned medium with different drug concentrations from days 0 and 6. d Effects of PTHrP-2 on migration of HUVECs, HFF-1 cells and HaCaTs and the tube formation assay of HUVECs. e Quantitation of HUVECs, HFF-1 cells and HaCaTs migration (violet stained cells) using a Transwell chamber. The quantitative evaluation of the number of nodes formed in the culture plate with different drug concentrations after 8 h. f Immunofluorescence images of HUVECs and HFF-1 incubated in each group on day 3. Cytoskeleton and cell nuclei are stained red and blue, VEGF and Collagen I are stained green in the picture taken by the laser scanning confocal microscopy. g VEGF and Collagen I secretion by HUVEC and HFF-1 incubated for 3 days in media with different drug concentrations. h Akt and Erk1/2 phosphorylation level in HUVEC and HFF-1 treated with different drug concentrations
Fig. 6a TEM images of HFF-1-Exos. b Exosome surface markers detected by Western blotting (Alix, Tsg101, CD9). The experiment was repeated three times in order to confirm the stability of the phenomena. c Size distribution of exosomes. Particle concentration, particle size and video frame of exosomes were analyzed by FNA (d) and NTA (e). Total protein levels (f) in HFF-1-Exos and PTHrP-2-HFF-1-Exos. g The uptake of exosomes by HUVECs and HFF-1 cells. Cytoskeleton, exosomes and cell nuclei are stained green, red and blue in the picture taken by the laser scanning confocal microscopy. h Proliferation of HUVECs and HFF-1 cells incubated for 0, 1, 3, or 7 days in conditioned medium with HFF-1-Exos and PTHrP-2-HFF-1-Exos from days 0 and 6. I Effects of HFF-1-Exos on migration of HUVECs and HFF-1 cells and the tube formation assay of HUVECs. j Quantitation of HUVECs and HFF-1 cells migration (violet stained cells) using a Transwell chamber. The quantitative evaluation of the number of nodes formed in the culture plate with different conditions of culture after 8 h