Eimad Dine Tariq Bouhlali1, Abdelbasset Hmidani2, Bouchra Bourkhis3, Tarik Khouya2, Hicham Harnafi4, Younes Filali-Zegzouti5, Chakib Alem2. 1. National Institute of Agronomic Research, Regional Center of Errachidia, 52000, Morocco; Biochemistry of Natural Products Team, Faculty of Sciences and Techniques Errachidia, 52000, Morocco. Electronic address: bouhlali.eimad@gmail.com. 2. Biochemistry of Natural Products Team, Faculty of Sciences and Techniques Errachidia, 52000, Morocco. 3. Faculty of Medicine and Pharmacy, Fes, 30070, Morocco. 4. Laboratory of Biochemistry and Biotechnologies, Faculty of Sciences, Oujda, 60000, Morocco. 5. Biology, Environment and Health Team, Faculty of Sciences and Techniques Errachidia, 50000, Morocco.
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE: Date seeds are widely used in Moroccan traditional medicine for treating obesity and related diseases. AIM OF THE STUDY: Two date seed varieties (Jihel and Majhoul) were assessed for their phenolic profile and lipid-lowering activity. MATERIALS AND METHODS: The polyphenolic profile was determined using HPLC-DAD. Triton-WR-1339 and high-fat diet (HFD)-induced hyperlipidaemic rats were used to evaluate the hypolipidaemic effect of date seeds extracts. Serum lipid profile was measured using automatic biochemical analyser. RESULTS: Rutin, quercetin, p-coumaric and caffeic acids were the most prevalent chemical among the analysed phenolic compounds. Serum lipids: Total cholesterol, triglycerides, low-density lipoprotein-cholesterol were lowered and high-density lipoprotein-cholesterol were increased by date seed methanolic extract (at 200 mg/kg) in triton WR1339 -induced hyperlipidaemia in experimental rats. Chronic feeding of these extracts (at 200 mg/kg), to animals concurrently fed with high fat diet (HFD) for three weeks, caused a significant (p < 0.05) decrease in serum total cholesterol, triglycerides, low-density lipoprotein-cholesterol levels and atherogenic index, while it increased serum HDL-C. Furthermore, the supplementation of date seed extract was effective in preventing body weight gain. CONCLUSIONS: These findings are suggestive of hypolipidaemic and atherosclerosis prevention roles of Jihel and Majhoul date seeds.
ETHNOPHARMACOLOGICAL RELEVANCE: Date seeds are widely used in Moroccan traditional medicine for treating obesity and related diseases. AIM OF THE STUDY: Two date seed varieties (Jihel and Majhoul) were assessed for their phenolic profile and lipid-lowering activity. MATERIALS AND METHODS: The polyphenolic profile was determined using HPLC-DAD. Triton-WR-1339 and high-fat diet (HFD)-induced hyperlipidaemic rats were used to evaluate the hypolipidaemic effect of date seeds extracts. Serum lipid profile was measured using automatic biochemical analyser. RESULTS:Rutin, quercetin, p-coumaric and caffeic acids were the most prevalent chemical among the analysed phenolic compounds. Serum lipids: Total cholesterol, triglycerides, low-density lipoprotein-cholesterol were lowered and high-density lipoprotein-cholesterol were increased by date seed methanolic extract (at 200 mg/kg) in triton WR1339 -induced hyperlipidaemia in experimental rats. Chronic feeding of these extracts (at 200 mg/kg), to animals concurrently fed with high fat diet (HFD) for three weeks, caused a significant (p < 0.05) decrease in serum total cholesterol, triglycerides, low-density lipoprotein-cholesterol levels and atherogenic index, while it increased serum HDL-C. Furthermore, the supplementation of date seed extract was effective in preventing body weight gain. CONCLUSIONS: These findings are suggestive of hypolipidaemic and atherosclerosis prevention roles of Jihel and Majhoul date seeds.
Authors: Jinmin Shi; Yifan Yang; Xinxin Zhou; Lijun Zhao; Xiaohua Li; Abdullah Yusuf; Mohaddeseh S M Z Hosseini; Fatemeh Sefidkon; Xuebo Hu Journal: Front Pharmacol Date: 2022-09-14 Impact factor: 5.988