Majid Salehi1,2, Saeed Farzamfar3, Arian Ehterami4, Zahrasadat Paknejad5,6, Farshid Bastami7,8, Sadegh Shirian9,10, Hamid Vahedi11, Gholamreza Savari Koehkonan12, Arash Goodarzi13. 1. Department of Tissue Engineering, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran. 2. Tissue Engineering and Stem Cell Research Center, Shahroud University of Medical Sciences, Shahroud, Iran. 3. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran. 4. Department of Mechanical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran. 5. Medical Nanotechnology and Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. 6. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. 7. Dental Research Center, Research Institute of Dental Sciences, School of Dentistry, Shahid Behest University of Medical Sciences, Tehran, Iran. 8. Oral and Maxillofacial Surgery Department, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran. 9. Department of Pathology, School of Veterinary Medicine, Shahrekord University, Shahrekord, Iran. 10. Shiraz Molecular Pathology Research Center, Dr. Daneshbod Pathology Lab, Shiraz, Iran. 11. Clinical Research Development Unit, Imam Hossein Hospital, Shahroud University of Medical Sciences, Shahroud, Iran. 12. Department of Medical Nanotechnology, Islamic Azad University Tehran branch, Tehran, Iran. 13. Department of Tissue Engineering, School of Medicine, Fasa University of Medical Sciences, Fasa, Iran.
Abstract
OBJECTIVE: To evaluate the application of a fabricated dressing containing kaolin for skin regeneration in a rat model of excisional wounds. METHOD: In the present study, kaolin was loaded into electrospun polyvinyl alcohol (PVA)/chitosan polymer blend to develop a composite nanofibrous dressing. To make the yarns, kaolin with weight ratio of 5% was added to PVA/chitosan polymer blend and subsequently formed into nanofibres using the electrospinning method. Scaffolds were evaluated for to their microstructure, mechanical properties, surface wettability, water vapour transmission rate, water-uptake capacity, blood uptake capacity, blood compatibility, microbial penetration test, the number of colonies, and cellular response with the L929 cell line. Rats with full-thickness excisional wounds were treated with kaolin-containing and kaolin-free dressings. RESULTS: The study showed that rats treated with the kaolin-incorporated mats demonstrated a significant closure to nearly 97.62±4.81% after 14 days compared with PVA/chitosan and the sterile gauze, which showed 86.15±8.11% and 78.50±4.22% of wound closure, respectively. The histopathological studies showed that in the PVA/chitosan/kaolin group, dense and regular collagen fibres were formed, while wounds treated with sterile gauze or PVA/chitosan scaffolds had random and loose collagen fibres. CONCLUSION: Our results show the potential applicability of PVA/chitosan/kaolin scaffolds as a wound care material.
OBJECTIVE: To evaluate the application of a fabricated dressing containing kaolin for skin regeneration in a rat model of excisional wounds. METHOD: In the present study, kaolin was loaded into electrospun polyvinyl alcohol (PVA)/chitosan polymer blend to develop a composite nanofibrous dressing. To make the yarns, kaolin with weight ratio of 5% was added to PVA/chitosan polymer blend and subsequently formed into nanofibres using the electrospinning method. Scaffolds were evaluated for to their microstructure, mechanical properties, surface wettability, water vapour transmission rate, water-uptake capacity, blood uptake capacity, blood compatibility, microbial penetration test, the number of colonies, and cellular response with the L929 cell line. Rats with full-thickness excisional wounds were treated with kaolin-containing and kaolin-free dressings. RESULTS: The study showed that rats treated with the kaolin-incorporated mats demonstrated a significant closure to nearly 97.62±4.81% after 14 days compared with PVA/chitosan and the sterile gauze, which showed 86.15±8.11% and 78.50±4.22% of wound closure, respectively. The histopathological studies showed that in the PVA/chitosan/kaolin group, dense and regular collagen fibres were formed, while wounds treated with sterile gauze or PVA/chitosan scaffolds had random and loose collagen fibres. CONCLUSION: Our results show the potential applicability of PVA/chitosan/kaolin scaffolds as a wound care material.
Authors: Tamer M Tamer; Maysa M Sabet; Mohamed A Hassan; Ahmed M Omer; Eman Abbas; Alaa I Eid; Mohamed S Mohy-Eldin Journal: Sci Rep Date: 2021-02-09 Impact factor: 4.379
Authors: Marwa H Gouda; Safaa M Ali; Sarah Samir Othman; Samia A Abd Al-Aziz; Marwa M Abu-Serie; Noha A Elsokary; Noha A Elessawy Journal: Sci Rep Date: 2021-10-14 Impact factor: 4.379