| Literature DB >> 32416340 |
Qiyi He1, Huiyi Yang2, Yingshan Chen3, Ding Shen4, Xiping Cui5, Chunguo Zhang6, Huanxin Xiao7, Sergei A Eremin8, Yanxiong Fang9, Suqing Zhao10.
Abstract
Prussian Blue nanoparticles (PBNPs) were utilized in a lateral flow immunoassay (LFA) and in an indirect competitive nanozyme-linked immunosorbent assay (icELISA), respectively, for their intense blue color and peroxidase (POx) -like activity. The PBNPs with good POx-like activity was linked to the antibody. Under the optimal parameters, both the PBNP-icELISA and PBNP-LFA perform very well. The icELISA has an IC50 value of 190 ng/mL, the working range extends from 29 to 1200 ng/mL, and the limit of detection is 22 ng/mL. The visual cut-off limit is 10 ng/mL. The dual immunoassay was used to quantify glycocholic acid in spiked human urine. Excellent recoveries and correlation between the two methods were observed.Entities:
Keywords: Glycocholic acid; Immunoassay; Lateral flow immunoassay; Nanozyme-linked immunosorbent assay; Prussian blue nanoparticles
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Year: 2020 PMID： 32416340 DOI： 10.1016/j.jpba.2020.113317
Source DB: PubMed Journal: J Pharm Biomed Anal ISSN： 0731-7085 Impact factor: 3.935