Quercetin alleviated H2 O2 -induced apoptosis and steroidogenic impairment in goat luteinized granulosa cells.

Quercetin (Que) is a natural flavonoid in most plants. Luteinized granulosa cell (LGC) culture is necessary for the study of follicle growth/differentiation. In the present study, we analyzed the role of Que in steroid production and apoptosis in hydrogen peroxide (H2 O2 )-treated goat LGCs. The results showed that treatment with H2 O2 induced apoptosis in goat LGCs, and treatment with Que decreased LGC apoptosis induced by H2 O2 (P < .05), accompanied with the different expressions of BAX, BCL-2, Caspase 3, and Cleaved caspase 3. Meanwhile, the messenger RNA expressions of nuclear factor erythroid 2 like 2 (Nrf2) and its downstream genes were upregulated with H2 O2 +Que treatment, accompanied by the increased cellular viability (P < .05). Furthermore, Que alleviated H2 O2 -induced reduction in the secretion of progesterone (P4 ) (P < .05); however, it had no effect on the secretion of estrogen (E2 ). Simultaneously, the expressions of StAR and P450scc were increased when treated with Que +H2 O2 , compared with the group treated with only H2 O2 (P < .05). In conclusion, it is observed that Que could alleviate the H2 O2 -induced apoptosis and steroidogenic impairment in goat LGCs, which might be mediated by the Nrf2 pathway.