| Literature DB >> 32410020 |
Ruofan Yu1,2, Myeong Chan Jo3, Weiwei Dang4,5.
Abstract
The replicative aging of the budding yeast, Saccharomyces cerevisiae, has been a useful model for dissecting the molecular mechanisms of the aging process. Traditionally, the replicative lifespan (RLS) is measured by manually dissecting mother cells from daughter cells, which is a very tedious process. Since 2012, several microfluidic systems have been developed to automate the dissection process, significantly accelerating RLS determination. Here, we describe a detailed protocol of RLS measurement using a ommercially available microfluidic system based on the HYAA chip design, which enables data collection of up to 8000 cells in a single experiment.Entities:
Keywords: Budding yeast; Microfluidics; Replicative aging; Replicative lifespan
Mesh：
Substances：
Year: 2020 PMID： 32410020 DOI： 10.1007/978-1-0716-0592-9_1
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745