OBJECTIVE: Patients undergoing bariatric surgery present long-term metabolic improvements and reduced type 2 diabetes risk, despite long-term weight regain. We hypothesized that part of these protective effects could be linked to altered gene expression in white adipose tissue (WAT). METHODS: Transcriptomic profiling by gene microarray was performed in abdominal subcutaneous WAT from women before (n = 50) and two (n = 49) and five (n = 38) years after Roux-en-Y gastric bypass (RYGB) surgery as well as in 28 age-matched nonoperated women. RESULTS: In the obese women, the average body weight decrease was 38 kg 2 years postsurgery followed by an 8 kg weight regain between 2 and 5 years. Most of the long-term changes in WAT gene expression occurred during the first 2 years. However, a subset of genes encoding proteins involved in inflammation displayed a continued decrease between baseline, 2 and 5 years, respectively; that is an expression pattern independent of body weight regain. Expression of 71 of these genes correlated with measurements of adipocyte morphology or serum adipokine levels. CONCLUSION: The continuous improvement in WAT inflammatory gene expression, despite body weight relapse, may contribute to the sustained effects on adipose morphology after bariatric surgery.
OBJECTIVE:Patients undergoing bariatric surgery present long-term metabolic improvements and reduced type 2 diabetes risk, despite long-term weight regain. We hypothesized that part of these protective effects could be linked to altered gene expression in white adipose tissue (WAT). METHODS: Transcriptomic profiling by gene microarray was performed in abdominal subcutaneous WAT from women before (n = 50) and two (n = 49) and five (n = 38) years after Roux-en-Y gastric bypass (RYGB) surgery as well as in 28 age-matched nonoperated women. RESULTS: In the obesewomen, the average body weight decrease was 38 kg 2 years postsurgery followed by an 8 kg weight regain between 2 and 5 years. Most of the long-term changes in WAT gene expression occurred during the first 2 years. However, a subset of genes encoding proteins involved in inflammation displayed a continued decrease between baseline, 2 and 5 years, respectively; that is an expression pattern independent of body weight regain. Expression of 71 of these genes correlated with measurements of adipocyte morphology or serum adipokine levels. CONCLUSION: The continuous improvement in WAT inflammatory gene expression, despite body weight relapse, may contribute to the sustained effects on adipose morphology after bariatric surgery.
Authors: Tammy Lo; Renuka S Haridas; Eleanor J M Rudge; Robert P Chase; Keyvan Heshmati; Elizabeth M Lucey; Alison M Weigl; Otatade J Iyoha-Bello; Chelsea O Ituah; Emily J Benjamin; Seth W McNutt; Leena Sathe; Leanna Farnam; Benjamin A Raby; Ali Tavakkoli; Damien C Croteau-Chonka; Eric G Sheu Journal: J Clin Endocrinol Metab Date: 2022-01-18 Impact factor: 6.134
Authors: Alastair G Kerr; Zuoneng Wang; Na Wang; Kelvin H M Kwok; Jutta Jalkanen; Alison Ludzki; Simon Lecoutre; Dominique Langin; Martin O Bergo; Ingrid Dahlman; Carsten Mim; Peter Arner; Hui Gao Journal: Nat Commun Date: 2022-05-26 Impact factor: 17.694
Authors: Birgitta W van der Kolk; Maheswary Muniandy; Dorota Kaminska; Marcus Alvarez; Arthur Ko; Zong Miao; Armand Valsesia; Dominique Langin; Maija Vaittinen; Mirva Pääkkönen; Riikka Jokinen; Sanna Kaye; Sini Heinonen; Kirsi A Virtanen; Daniel P Andersson; Ville Männistö; Wim H Saris; Arne Astrup; Mikael Rydén; Ellen E Blaak; Päivi Pajukanta; Jussi Pihlajamäki; Kirsi H Pietiläinen Journal: J Clin Endocrinol Metab Date: 2021-04-23 Impact factor: 5.958