Ying Guan1, Feng Sun2, Xiaojuan Zhang1, Zhibin Peng1, Bo Jiang1, Min Liang1, Yansong Wang3. 1. Department of Orthopedic Surgery, The First Affiliated Hospital of Harbin Medical University, 150001, Harbin, China. 2. Department of Orthopedic Surgery, General Hospital of Heilongjiang Province Land Reclamation Headquarter, 150001, Harbin, China. 3. Department of Orthopedic Surgery, The First Affiliated Hospital of Harbin Medical University, 150001, Harbin, China. yansongwang168@163.com.
Abstract
BACKGROUND: Skin injury is a kind of common tissue damage in daily life and war. Silk fibroin (SF) is becoming an engineered material for skin wound repair due to its superior unique physical and chemical properties. The present study aimed to illustrate mechanism of SF hydrogel promoting skin repair in the second degree burn mice. METHODS: Heat shock models were established. In vitro, cells were culture for 50 min at 44 °C water bath; while in vivo, the skin of anesthetic mice were treat with soldering iron at 90 °C. Then, they divided into silk fibroin gel group, purilon gel group and control (blank) group. The cellular activity of proliferation and apoptosis was detected by Kit-8, flow cytometry and HE-staining, and the migration and adhesion were detected by scratch test. qRT-PCR and WB were employed to detected adhesion and migration related genes and proteins expression. TLN1 siRNA and overexpression technologies were also employed to illustrate the potential mechanism of SF effects. RESULTS: Compared with the purilon gel group and control group, SF hydrogel could enhance cell proliferation, migration and adhesion and increase the expression of adhesion and migration related proteins (P < 0.05), which promote burn wound healing. CONCLUSIONS: Through the inhibition, overexpression and rescue experiments of Talin1, we proved that silk fibroin hydrogel promote burn wound healing through regulating TLN1 expression and affecting cell adhesion and migration.
BACKGROUND:Skin injury is a kind of common tissue damage in daily life and war. Silk fibroin (SF) is becoming an engineered material for skin wound repair due to its superior unique physical and chemical properties. The present study aimed to illustrate mechanism of SF hydrogel promoting skin repair in the second degree burn mice. METHODS: Heat shock models were established. In vitro, cells were culture for 50 min at 44 °C water bath; while in vivo, the skin of anesthetic mice were treat with soldering iron at 90 °C. Then, they divided into silk fibroin gel group, purilon gel group and control (blank) group. The cellular activity of proliferation and apoptosis was detected by Kit-8, flow cytometry and HE-staining, and the migration and adhesion were detected by scratch test. qRT-PCR and WB were employed to detected adhesion and migration related genes and proteins expression. TLN1 siRNA and overexpression technologies were also employed to illustrate the potential mechanism of SF effects. RESULTS: Compared with the purilon gel group and control group, SF hydrogel could enhance cell proliferation, migration and adhesion and increase the expression of adhesion and migration related proteins (P < 0.05), which promote burn wound healing. CONCLUSIONS: Through the inhibition, overexpression and rescue experiments of Talin1, we proved that silk fibroin hydrogel promote burn wound healing through regulating TLN1 expression and affecting cell adhesion and migration.