Literature DB >> 32405756

Enzymatic removal of dags from livestock: an agricultural application of enzyme technology.

Laura Navone1, Robert Speight2.   

Abstract

The effective removal of dags (manure balls) from cattle, sheep and goats is a significant issue for the livestock industry. Dags are hard recalcitrant deposits composed of materials, such as faeces, hair, soil, urine, feed and straw, and attach to the animal through the hair rather than the skin. Dags build up during wet periods, especially on long haired breeds, and can weigh up to 40 kg per animal for cattle. Dag removal prior to slaughter reduces the risk of microbial meat contamination and damage to the hide during leather processing. Existing removal methods include hair trimming or extensive hose washing that can result in stress to the animal and increased costs. An alternative solution is the application of enzyme formulations that target specific components of the dag so they are more easily removed by washing. Enzymes are already used in other cleaning applications and are proven for the breakdown of materials such as lignocellulose, protein or starch that are found in dags. This mini-review discusses the challenges of current dag removal methods and the state of the art and feasibility of applying enzyme formulations for the effective removal of dags. Although enzyme formulations are yet to be tested in large-scale cattle trials and questions remain regarding how they would be cost-effectively applied to live animals, the results at laboratory scale suggest further research is warranted. Overall, enzymes present an environmentally friendly solution to the high costs and animal welfare issues of current dag removal methods through significant reductions in cleaning time and water use. KEY POINTS: • Dag formation on livestock is a major issue for industry and for animal welfare. • Current methods are costly and challenging for operators and the animal. • Enzymes can degrade dag components to aid release with keratinases showing promise. • Dag removal needs to be field tested, and positive business cases must be generated.

Entities:  

Keywords:  Biomass; Dag; Enzymes; Keratinases; Livestock

Mesh:

Substances:

Year:  2020        PMID: 32405756     DOI: 10.1007/s00253-020-10656-2

Source DB:  PubMed          Journal:  Appl Microbiol Biotechnol        ISSN: 0175-7598            Impact factor:   4.813


  25 in total

Review 1.  Microbial keratinases and their prospective applications: an overview.

Authors:  Rani Gupta; Priya Ramnani
Journal:  Appl Microbiol Biotechnol       Date:  2006-01-04       Impact factor: 4.813

2.  Molecular packing in the feather keratin filament.

Authors:  R D Bruce Fraser; David A D Parry
Journal:  J Struct Biol       Date:  2008-02-02       Impact factor: 2.867

Review 3.  A current assessment on the production of bacterial keratinases.

Authors:  Daniel Joner Daroit; Adriano Brandelli
Journal:  Crit Rev Biotechnol       Date:  2013-08-13       Impact factor: 8.429

Review 4.  Biotechnological applications and prospective market of microbial keratinases.

Authors:  Rani Gupta; Rinky Rajput; Richa Sharma; Namita Gupta
Journal:  Appl Microbiol Biotechnol       Date:  2013-10-13       Impact factor: 4.813

Review 5.  Biochemical features of microbial keratinases and their production and applications.

Authors:  Adriano Brandelli; Daniel J Daroit; Alessandro Riffel
Journal:  Appl Microbiol Biotechnol       Date:  2009-12-29       Impact factor: 4.813

6.  Keratinous waste decomposition and peptide production by keratinase from Geobacillus stearothermophilus AD-11.

Authors:  Audrius Gegeckas; Renata Gudiukaitė; Janusz Debski; Donaldas Citavicius
Journal:  Int J Biol Macromol       Date:  2015-01-24       Impact factor: 6.953

7.  Structural basis for the enhancement of virulence by viral spindles and their in vivo crystallization.

Authors:  Elaine Chiu; Marcel Hijnen; Richard D Bunker; Marion Boudes; Chitra Rajendran; Kaheina Aizel; Vincent Oliéric; Clemens Schulze-Briese; Wataru Mitsuhashi; Vivienne Young; Vernon K Ward; Max Bergoin; Peter Metcalf; Fasséli Coulibaly
Journal:  Proc Natl Acad Sci U S A       Date:  2015-03-18       Impact factor: 11.205

8.  Correlation of the mechanical and structural properties of cortical rachis keratin of rectrices of the Toco Toucan (Ramphastos toco).

Authors:  S G Bodde; M A Meyers; J McKittrick
Journal:  J Mech Behav Biomed Mater       Date:  2011-02-04

9.  The effects of preslaughter washing on the reduction of Escherichia coli O157:H7 transfer from cattle hides to carcasses during slaughter.

Authors:  C M Byrne; D J Bolton; J J Sheridan; D A McDowell; I S Blair
Journal:  Lett Appl Microbiol       Date:  2000-02       Impact factor: 2.858

10.  Distribution and sources of microbial contamination on beef carcasses.

Authors:  R G Bell
Journal:  J Appl Microbiol       Date:  1997-03       Impact factor: 3.772

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