Evaluation of the protein and bioactive compound bioaccessibility/bioavailability and cytotoxicity of the extracts obtained from aquaculture and fisheries by-products.

Bioavailability, bioaccessibility, bioactivity and cytotoxicity define if a bioactive compound obtained from aquaculture and associated by-products can be assimilated and used for the body in a safe and efficient way. Four models are used to evaluate the bioavailability: in vitro (simulated gastrointestinal digestion using intestinal epithelial Caco-2 cell cultures); ex vivo (gastrointestinal organs or organoids in laboratory conditions); in situ (intestinal perfusion in animals) and in vivo (animal studies and human studies). In vitro models are very effective, predicting in vivo actions since they evaluate multiple conditions regardless physiological effects. However, in vivo systems are essential for the validation of the results. The use of a combined model between human digestion and cell culture-based models would solve these difficulties, allowing valid conclusions. These studies must be completed with the evaluation of cytotoxicity and oxidative stress markers, providing most accurate results regarding the adverse effect on the body. These methods would test the effect of food structure, food composition, dietary factors and the effect of food processing on bioavailability. Further studies should be carried out to establish a standardized method and achieve a balance between the use of in vivo and in vitro systems.