| Literature DB >> 32401505 |
Jinghua Huang1,2,3, Qibin Ma1,2,3, Zhandong Cai1,2,3, Qiuju Xia4, Shuxian Li5, Jia Jia1,2,3, Li Chu1,2,3, Tengxiang Lian1,2,3, Hai Nian1,2,3, Yanbo Cheng1,2,3.
Abstract
This research aimed to identify stable quantitative trait loci (QTL) associated with oil and protein content in soybean. A population of 196 recombinant inbred lines (RILs) derived from Huachun 2 × Wayao was used to evaluate these target traits. A high-density genetic linkage map was constructed by using high-throughput genome-wide sequencing technology, which contained 3413 recombination bin markers and spanned 5400.4 cM with an average distance of 1.58 cM between markers. Eighteen stable QTLs controlling oil and protein content were detected. Among them, qOil-11-1 was identified for the first time as a novel QTL, while qOil-5-1, qPro-10-1, and qPro-14-1 were strong and stable QTLs with high log-likelihood (LOD) values. Sixteen differentially expressed genes (DEGs) within these four QTLs were shown to be highly expressed during seed development based on RNA sequencing (RNA-seq) data analysis. Our results may contribute toward gene mining and marker-assisted selection (MAS) for breeding a high-quality soybean in the future.Entities:
Keywords: QTL mapping; high-density genetic map; oil content; protein content; soybean
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Year: 2020 PMID： 32401505 DOI： 10.1021/acs.jafc.0c01271
Source DB: PubMed Journal: J Agric Food Chem ISSN： 0021-8561 Impact factor: 5.279