Gabriel Cara-Fuentes1, Madhusudan Venkatareddy2, Rakesh Verma2, Alfons Segarra3, Audrey C Cleuren4, Alfonso Martínez-Ramos5, Richard J Johnson6, Puneet Garg2. 1. Division of Pediatric Nephrology, Department of Pediatrics, University of Michigan, MSRB-2, Room 1574, 1500 E Medical Center Dr, Ann Arbor, MI, 48109, USA. gcaraf@gmail.com. 2. Division of Nephrology, Department of Internal Medicine, University of Michigan, Ann Arbor, USA. 3. Division of Nephrology, Hospital Vall d'Hebron, Barcelona, Spain. 4. Life Sciences Institute, University of Michigan, Ann Arbor, USA. 5. School of Medicine, University of Granada, Granada, Spain. 6. Division of Renal Diseases and Hypertension, Department of Medicine, University of Colorado, Denver, USA.
Abstract
BACKGROUND: Urinary CD80 has emerged as potential biomarker in idiopathic nephrotic syndrome (INS). However, its cellular source remains controversial. The aim of the study was to assess whether CD80 is truly expressed by glomerular cells in INS patients during relapse and in the LPS mouse model of podocyte injury. METHODS: The presence of CD80 in glomeruli was evaluated by combining immunostaining, immunogold labeling, and in situ hybridization techniques. RESULTS: CD80 was present along the surface of glomerular endothelial cells (GEC) and rarely in podocytes in six of nine minimal change disease (MCD) patients in relapse, two of eleven patients with focal segmental glomerulosclerosis in relapse, and absent in controls. In mice, CD80 was upregulated at mRNA and protein level in GEC and podocytes, in a similar pattern to that seen in MCD patients. CONCLUSIONS: Glomerular endothelial cells and podocytes can express CD80 in patients with MCD during relapse. A better understanding of the role of CD80 in glomerular cells may provide further insights into the mechanisms of proteinuria in INS.
BACKGROUND: Urinary CD80 has emerged as potential biomarker in idiopathic nephrotic syndrome (INS). However, its cellular source remains controversial. The aim of the study was to assess whether CD80 is truly expressed by glomerular cells in INS patients during relapse and in the LPS mouse model of podocyte injury. METHODS: The presence of CD80 in glomeruli was evaluated by combining immunostaining, immunogold labeling, and in situ hybridization techniques. RESULTS:CD80 was present along the surface of glomerular endothelial cells (GEC) and rarely in podocytes in six of nine minimal change disease (MCD) patients in relapse, two of eleven patients with focal segmental glomerulosclerosis in relapse, and absent in controls. In mice, CD80 was upregulated at mRNA and protein level in GEC and podocytes, in a similar pattern to that seen in MCDpatients. CONCLUSIONS: Glomerular endothelial cells and podocytes can express CD80 in patients with MCD during relapse. A better understanding of the role of CD80 in glomerular cells may provide further insights into the mechanisms of proteinuria in INS.
Authors: Colin Bauer; Federica Piani; Mindy Banks; Flor A Ordoñez; Carmen de Lucas-Collantes; Kaori Oshima; Eric P Schmidt; Igor Zakharevich; Alfons Segarra; Cristina Martinez; Carlos Roncal-Jimenez; Simon C Satchell; Petter Bjornstad; Marshall Scott Lucia; Judith Blaine; Joshua M Thurman; Richard J Johnson; Gabriel Cara-Fuentes Journal: Kidney Int Rep Date: 2021-12-16
Authors: Anatilde M Gonzalez Guerrico; John Lieske; George Klee; Sanjay Kumar; Victor Lopez-Baez; Adam M Wright; Shane Bobart; Diane Shevell; Michael Maldonado; Jonathan P Troost; Marie C Hogan Journal: Kidney Int Rep Date: 2020-08-14
Authors: Shrey Purohit; Federica Piani; Flor A Ordoñez; Carmen de Lucas-Collantes; Colin Bauer; Gabriel Cara-Fuentes Journal: Front Med (Lausanne) Date: 2021-12-23
Authors: Gabriel Cara-Fuentes; Ana Andres-Hernando; Colin Bauer; Mindy Banks; Gabriela E Garcia; Christina Cicerchi; Masanari Kuwabara; Michiko Shimada; Richard J Johnson; Miguel A Lanaspa Journal: iScience Date: 2022-06-30