Literature DB >> 3239778

Colorimetric determination of neutral sugars by a resorcinol sulfuric acid micromethod.

M Monsigny1, C Petit, A C Roche.   

Abstract

A microscale colorimetric assay for neutral sugars, in which neutral sugars react with resorcinol in the presence of 75% sulfuric acid solution is described. This assay, based on the use of microtiter plates with 96 U-shaped wells is simple and easy to handle; it allows accurate determinations with small samples (20 microliters) containing 1 to 100 nmol of neutral sugars and is quite convenient for detection of glycoconjugates in chromatographic column effluents.

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Year:  1988        PMID： 3239778     DOI： 10.1016/0003-2697(88)90578-7

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  45 in total

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