Literature DB >> 3239751

A simple method for DNA purification from peripheral blood.

T A Ciulla1, R M Sklar, S L Hauser.   

Abstract

A new, simple, and inexpensive method for the rapid isolation of DNA from whole blood is described. Cell nuclei are prepared by lysis of cytoplasmic membranes and DNA within the nuclear pellet is dispersed with guanidine isothiocyanate and precipitated with isopropanol. DNA prepared in this way restricts completely and results in low backgrounds of nonspecific hybridization after Southern analysis. The yields of DNA are similar to those obtained by more tedious traditional procedures. Numerous genomic DNA samples can be prepared from whole blood in 2 h, thus facilitating gene linkage or other molecular studies in which large numbers of individuals are required.

Entities:  

Mesh：

Substances：

Year:  1988        PMID： 3239751     DOI： 10.1016/0003-2697(88)90047-4

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  45 in total

1.  Arg506Gln mutation of the coagulation factor V gene not detected in Japanese pulmonary thromboembolism.

Authors:  T Seki; H Okayama; T Kumagai; N Kumasaka; M Sakuma; S Isoyama; K Shirato; H Odaka
Journal:  Heart Vessels       Date:  1998       Impact factor: 2.037

2.  Whole genome analysis of genetic alterations in small DNA samples using hyperbranched strand displacement amplification and array-CGH.

Authors:  José M Lage; John H Leamon; Tanja Pejovic; Stefan Hamann; Michelle Lacey; Deborah Dillon; Richard Segraves; Bettina Vossbrinck; Antonio González; Daniel Pinkel; Donna G Albertson; Jose Costa; Paul M Lizardi
Journal:  Genome Res       Date:  2003-02       Impact factor: 9.043

3.  A genomewide search using an original pairwise sampling approach for large genealogies identifies a new locus for total and low-density lipoprotein cholesterol in two genetically differentiated isolates of Sardinia.

Authors:  Mario Falchi; Paola Forabosco; Evelina Mocci; Cesare Cappio Borlino; Andrea Picciau; Emanuela Virdis; Ivana Persico; Debora Parracciani; Andrea Angius; Mario Pirastu
Journal:  Am J Hum Genet       Date:  2004-10-11       Impact factor: 11.025

4.  Microcephaly, intellectual impairment, bilateral vesicoureteral reflux, distichiasis, and glomuvenous malformations associated with a 16q24.3 contiguous gene deletion and a Glomulin mutation.

Authors:  Matthew G Butler; Susan L Dagenais; José L Garcia-Perez; Pascal Brouillard; Miikka Vikkula; Peter Strouse; Jeffrey W Innis; Thomas W Glover
Journal:  Am J Med Genet A       Date:  2012-03-09       Impact factor: 2.802

5.  Apolipoprotein E genotype and the association between C-reactive protein and postoperative delirium: Importance of gene-protein interactions.

Authors:  Sarinnapha M Vasunilashorn; Long H Ngo; Sharon K Inouye; Tamara G Fong; Richard N Jones; Simon T Dillon; Towia A Libermann; Margaret O'Connor; Steven E Arnold; Zhongcong Xie; Edward R Marcantonio
Journal:  Alzheimers Dement       Date:  2020-01-04       Impact factor: 21.566

6.  Ocular refraction: heritability and genome-wide search for eye morphometry traits in an isolated Sardinian population.

Authors:  Ginevra Biino; Maria Antonietta Palmas; Carla Corona; Dionigio Prodi; Manuela Fanciulli; Roberta Sulis; Antonina Serra; Maurizio Fossarello; Mario Pirastu
Journal:  Hum Genet       Date:  2004-12-21       Impact factor: 4.132

7.  Modified salting-out method: high-yield, high-quality genomic DNA extraction from whole blood using laundry detergent.

Authors:  H Nasiri; M Forouzandeh; M J Rasaee; F Rahbarizadeh
Journal:  J Clin Lab Anal       Date:  2005       Impact factor: 2.352

8.  Rapid and simple method for purification of nucleic acids.

Authors:  R Boom; C J Sol; M M Salimans; C L Jansen; P M Wertheim-van Dillen; J van der Noordaa
Journal:  J Clin Microbiol       Date:  1990-03       Impact factor: 5.948

9.  A new essential hypertension susceptibility locus on chromosome 2p24-p25, detected by genomewide search.

Authors:  Andrea Angius; Enrico Petretto; Giovanni Battista Maestrale; Paola Forabosco; Giuseppina Casu; Daniela Piras; Manuela Fanciulli; Mario Falchi; Paola Maria Melis; Mario Palermo; Mario Pirastu
Journal:  Am J Hum Genet       Date:  2002-09-12       Impact factor: 11.025

10.  Loss of heterozygosity on chromosome 11 in sporadic gastrinomas.

Authors:  M P Sawicki; Y J Wan; C L Johnson; J Berenson; R Gatti; E Passaro
Journal:  Hum Genet       Date:  1992-06       Impact factor: 4.132
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.