| Literature DB >> 32394387 |
Leah K Officer1, Kleopatra E Andreou1, Ana V Teodósio1, Zhangyi He1, John P Le Quesne2,3,4.
Abstract
In situ hybridization (ISH) and immunohistochemistry (IHC) are valuable tools for molecular pathology and cancer research. Recent advances in multiplex technology, assay automation, and digital image analysis have enabled the development of co-ISH IHC or immunofluorescence (IF) methods, which allow researchers to simultaneously view and quantify expression of mRNA and protein within the preserved tissue spatial context. These data are vital to the study of the control of gene expression in the complex tumor microenvironment.Entities:
Keywords: Autostainer; Digital pathology; Image analysis; Immunofluorescence; Immunohistochemistry; In situ hybridization; Multiplex; Pathology informatics; Quantitative analysis; Translational control
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Year: 2020 PMID： 32394387 DOI： 10.1007/978-1-0716-0623-0_16
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745