| Literature DB >> 32391143 |
Yi Wang1, Zhiheng Guo1, Yongmei Gao1, Ping Liang1, Yanhong Shan1, Jin He1.
Abstract
[This corrects the article DOI: 10.1186/s13578-019-0348-1.].Entities:
Year: 2020 PMID: 32391143 PMCID: PMC7199318 DOI: 10.1186/s13578-020-00419-x
Source DB: PubMed Journal: Cell Biosci ISSN: 2045-3701 Impact factor: 7.133
Fig. 1LCZ696 alleviates cardiac injury in PAH mice and represses the Ang II receptor pathway. PAH mice were either treated with or without LCZ696. a Effect of LCZ696 on survival rate of PAH mice detected by Kaplan–Meier method (N = 7). b Heart size (scale bar = 100 mm) and ratio of heart weight/body weight of mice. c HE staining analysis of cardiac tissues (upper panels: scale bar = 50 mm; lower panels: bar = 25 μm). d Cardiac fibrosis observed by Masson’s trichrome staining (scale bar = 25 μm). e Apoptosis of cardiomyocytes detected by TUNEL staining (scale bar = 25 μm). f Western blot analysis of ACE2 protein. N = 7. *p < 0.05 vs. normal mice; #p < 0.05 vs. PAH mice. Measurement data (mean ± S.D.) among multiple groups were analyzed by one-way ANOVA, followed by Tukey post hoc test. Survival rate was calculated by the Kaplan–Meier method, and compared by a log-rank test
Fig. 5LCZ696 reduces cardiac remodeling through inhibiting the ERK signaling pathway. PAH mice were treated with sh-ERK with sh-NC as the control, or sh-ERK in the presence of LCZ696 with sh-NC in the presence of LCZ696 or control. a Heart size (scale bar = 100 mm) and ratio of heart weight/body weight in PAH mice. b HE staining of ventricular transection of heart (upper panels: scale bar = 50 mm; lower panels: scale bar = 25 μm). c Cardiomyocyte fibrosis observed by Masson’s trichrome staining (scale bar = 25 μm). d mRNA expression of collagen I, collagen III and TGF-β detected by RT-qPCR. e Isolectin B4 immunofluorescence of the capillary in ventricular tissues (blood vessel: yellow; cell membrane: red; nucleus: blue). f mRNA expression of ANP, βMHC and TIMP2 in cardiomyocytes detected by RT-qPCR. g Apoptosis of cardiomyocytes treated with LCZ696 detected by TUNEL staining (scale bar = 25 μm). h Western blot analysis of ERK protein and extent of ERK phosphorylation. *p < 0.05 vs. PAH mice treated with sh-NC; #p < 0.05 vs. PAH mice treated with sh-ERK; &p < 0.05 vs. PAH mice treated with sh-NC + LCZ696. N = 7. Measurement data (mean ± S.D.) among multiple groups was analyzed by one-way ANOVA, followed by Tukey post hoc test