Bo Zheng1, Minghua Xue2, Xinyi Zhang1, Ning Tian1, Dongmei Wang1. 1. Department of Medical Imaging, Shandong Provincial Third Hospital, Jinan 250031, Shandong, PR China. 2. Department of Ultrasonography, Shandong Provincial Qianfoshan Hospital, The First Hospital Affiliated with Shandong First Medical Univerisity, Jinan 250014, Shandong, PR China.
Abstract
Objective: This study aimed to determine the effects of dimer captosuccinic acid-coated Fe₃O₄ (super paramagnetic) nanoparticles (NP) on 2-deoxy-d-glucose in targeted cancer cells with high rates of glucose metabolism. Methods: We prepared Fe₃O₄@DMSA NP and 2-DG-conjugated Fe₃O₄@DMSA NP, γ-FE, O, and @DMSA-DG NP. Glucose consumption in MDA-MB-231 and MCF-7 breast cancer cells was determined using γ-Fe2O3@DMSA NP or Fe₃O₄@DMSA-DG NP, and absorption was tested using Prussian blue staining, ultraviolet colorimetry, and magnetic resonance imaging. Results: Glucose consumption was the highest in MDA-MB-231, and the lowest in human mammary epithelial cells (HMEPiC). The significant uptake of Fe2O3@DMSA-DG NP by MDA-MB-231 and MCF-7 cells within two hours was inhibited by glucose. The uptake of Fe₃O₄@DMSA-DG NP was significantly higher in MDA-MB-231 than in MCF-7 cells, whereas Fe₃O₄@DMSA NP was not obviously uptaken by either cell line. Absorption was also not evident in HMEPiC incubated with Fe₃O₄@DMSA-DG NP and Fe₃O₄@DMSA NP. Conclusions: The tumor targeting efficacy of 2-DG coated Fe₃O₄@DMSA NP was improved over Fe₃O₄,@DMSA NP in cancer cells with high rates of glucose metabolism.
Objective: This study aimed to determine the effects of dimer captosuccinic acid-coated Fe₃O₄ (super paramagnetic) nanoparticles (NP) on 2-deoxy-d-glucose in targeted cancer cells with high rates of glucose metabolism. Methods: We prepared Fe₃O₄@DMSA NP and 2-DG-conjugated Fe₃O₄@DMSA NP, γ-FE, O, and @DMSA-DG NP. Glucose consumption in MDA-MB-231 and MCF-7 breast cancer cells was determined using γ-Fe2O3@DMSA NP or Fe₃O₄@DMSA-DG NP, and absorption was tested using Prussian blue staining, ultraviolet colorimetry, and magnetic resonance imaging. Results:Glucose consumption was the highest in MDA-MB-231, and the lowest in human mammary epithelial cells (HMEPiC). The significant uptake of Fe2O3@DMSA-DG NP by MDA-MB-231 and MCF-7 cells within two hours was inhibited by glucose. The uptake of Fe₃O₄@DMSA-DG NP was significantly higher in MDA-MB-231 than in MCF-7 cells, whereas Fe₃O₄@DMSA NP was not obviously uptaken by either cell line. Absorption was also not evident in HMEPiC incubated with Fe₃O₄@DMSA-DG NP and Fe₃O₄@DMSA NP. Conclusions: The tumor targeting efficacy of 2-DG coated Fe₃O₄@DMSA NP was improved over Fe₃O₄,@DMSA NP in cancer cells with high rates of glucose metabolism.