Carmen Gómez1, Doris Salcedo-Moncada2, Gerardo Ayala2, Romel Watanabe2, Martha Pineda2, Daniel Alvítez-Temoche2, Frank Mayta-Tovalino3. 1. Faculty of Dentistry, Universidad Nacional Mayor de San Marcos, Lima, Peru. 2. Academic Department of Rehabilitative Stomatology, Faculty of Dentistry, Universidad Nacional Mayor de San Marcos, Lima, Peru. 3. Academic Department of Rehabilitative Stomatology, Faculty of Dentistry, Universidad Nacional Mayor de San Marcos, Lima, Peru; Postgrade Department, Faculty of Health Sciences, Universidad Científica del Sur, Lima, Peru, Phone: +51 1 214-2500, e-mail: fmaytat@ucientifica.edu.pe.
Abstract
AIM: To compare the antimicrobial efficacy of sodium hypochlorite (NaClO at 2.5% and 5.25%) and calcium hypochlorite [Ca(ClO)2 at 2.5%] on a biofilm of Enterococcus faecalis ATCC 29212™ and Candida albicans ATCC 10231™. MATERIALS AND METHODS: We performed an experimental in vitro study. Strains of C. albicans and E. faecalis, which had previously been reactivated were used. Then the colonies to be used were standardized in a turbidity standard to guarantee a quantity of 108 (CFU/mL) using the McFarland scale (0.5). Subsequently, the biofilm formed in brain-heart infusion agar was seeded into 42 sterile disks previously embedded with the experimental substances. Both 2.5% NaClO and Ca(ClO)2 solutions were placed in each Petri dish. They were then incubated at 37°C for 24 hours and the inhibition halos were measured using the Kirby-Bauer technique. RESULTS: The means between the halos corresponding to NaClO and Ca(ClO)2 at 2.5% were 13.38 ± 0.64 mm and 13.42 ± 0.62 mm, respectively. According to the Tukey test, no statistically significant differences were found between the hypochlorite groups evaluated (p = 0.989). CONCLUSION: Both Ca(ClO)2 and NaClO have a similar antimicrobial efficacy with biofilm based on E. faecalis and C. albicans, with no statistically significant differences between the two. CLINICAL SIGNIFICANCE: This study demonstrates the effectiveness of Ca(ClO)2 and NaClO as endodontic irrigators to combat the most frequent microorganisms of the root canal.
AIM: To compare the antimicrobial efficacy of sodium hypochlorite (NaClO at 2.5% and 5.25%) and calcium hypochlorite [Ca(ClO)2 at 2.5%] on a biofilm of Enterococcus faecalis ATCC 29212™ and Candida albicans ATCC 10231™. MATERIALS AND METHODS: We performed an experimental in vitro study. Strains of C. albicans and E. faecalis, which had previously been reactivated were used. Then the colonies to be used were standardized in a turbidity standard to guarantee a quantity of 108 (CFU/mL) using the McFarland scale (0.5). Subsequently, the biofilm formed in brain-heart infusion agar was seeded into 42 sterile disks previously embedded with the experimental substances. Both 2.5% NaClO and Ca(ClO)2 solutions were placed in each Petri dish. They were then incubated at 37°C for 24 hours and the inhibition halos were measured using the Kirby-Bauer technique. RESULTS: The means between the halos corresponding to NaClO and Ca(ClO)2 at 2.5% were 13.38 ± 0.64 mm and 13.42 ± 0.62 mm, respectively. According to the Tukey test, no statistically significant differences were found between the hypochlorite groups evaluated (p = 0.989). CONCLUSION: Both Ca(ClO)2 and NaClO have a similar antimicrobial efficacy with biofilm based on E. faecalis and C. albicans, with no statistically significant differences between the two. CLINICAL SIGNIFICANCE: This study demonstrates the effectiveness of Ca(ClO)2 and NaClO as endodontic irrigators to combat the most frequent microorganisms of the root canal.
Authors: Betty Cabanillas; Adrián Mallma-Medina; Marieta Petkova-Gueorguieva; Daniel Alvitez-Temoche; Román Mendoza; Frank Mayta-Tovalino Journal: J Int Soc Prev Community Dent Date: 2021-01-30