Kadhiresan Rathinasamy1, Arunmozhi Ulaganathan2, Shanmugapriya Ramamurthy2, Rathinavelu Ganesan2, Parthiban Saket3, Swarna Alamelu4. 1. Department of Periodontology and Oral Implantology, Sri Venkateswara Dental College and Hospital, Chennai, Tamil Nadu, India, Phone: +91 9840743339, e-mail: drkadhir@yahoo.com. 2. Department of Periodontology and Oral Implantology, Sri Venkateswara Dental College and Hospital, Chennai, Tamil Nadu, India. 3. Department of Periodontology and Oral Implantology, Adhiparasakthi Dental College and Hospital, Melmaruvathur, Tamil Nadu, India. 4. Department of Periodontology and Oral Implantology, Ragas Dental College, Chennai, Tamil Nadu, India.
Abstract
AIM: The aim of this case-control study is to estimate the circulatory levels of tumor necrosis factor alpha (TNF-α) in saliva and serum of patients with chronic periodontitis and periodontally healthy subjects. MATERIALS AND METHODS: Forty-four patients were screened, and based on biofilm-gingival interface (BGI) index, they were grouped into group I healthy periodontium [BGI-H (20)] and group II periodontitis [BGI-P3 (24)]. Venous blood and salivary samples were collected and analyzed using solid-phase enzyme-linked immunosorbent assay. Independent sample t test was performed to determine the association. RESULTS: Overall, there were differences in both the saliva and the serum TNF-α levels in healthy and periodontitis subjects. The average serum TNF-α concentration in group I healthy subjects was 23.12 pg/mL and in group II periodontitis was 24.06 pg/mL. In the saliva, the mean TNF-α level in group I healthy subjects was 45.69 pg/mL and in group II diseased subjects was 46.58 pg/mL. However, the values were not statistically significant (p > 0.05). CONCLUSION: Circulatory and salivary TNF-α levels were found in detectable quantities. They showed a marginal increase in chronic periodontitis patients when compared with normal healthy patients in the absence of systemic diseases. Further studies are required in a large scale and with different methodologies to substantiate the role of TNF-α in the progression of periodontal diseases. CLINICAL SIGNIFICANCE: Clinical significance of this study is to analyze the TNF-α levels in saliva and serum, which may be the aggravating factor in causing periodontal disease, thereby helping to treat periodontitis.
AIM: The aim of this case-control study is to estimate the circulatory levels of tumor necrosis factor alpha (TNF-α) in saliva and serum of patients with chronic periodontitis and periodontally healthy subjects. MATERIALS AND METHODS: Forty-four patients were screened, and based on biofilm-gingival interface (BGI) index, they were grouped into group I healthy periodontium [BGI-H (20)] and group II periodontitis [BGI-P3 (24)]. Venous blood and salivary samples were collected and analyzed using solid-phase enzyme-linked immunosorbent assay. Independent sample t test was performed to determine the association. RESULTS: Overall, there were differences in both the saliva and the serum TNF-α levels in healthy and periodontitis subjects. The average serum TNF-α concentration in group I healthy subjects was 23.12 pg/mL and in group II periodontitis was 24.06 pg/mL. In the saliva, the mean TNF-α level in group I healthy subjects was 45.69 pg/mL and in group II diseased subjects was 46.58 pg/mL. However, the values were not statistically significant (p > 0.05). CONCLUSION: Circulatory and salivary TNF-α levels were found in detectable quantities. They showed a marginal increase in chronic periodontitispatients when compared with normal healthy patients in the absence of systemic diseases. Further studies are required in a large scale and with different methodologies to substantiate the role of TNF-α in the progression of periodontal diseases. CLINICAL SIGNIFICANCE: Clinical significance of this study is to analyze the TNF-α levels in saliva and serum, which may be the aggravating factor in causing periodontal disease, thereby helping to treat periodontitis.