Mingyang Jian1, Dazhong Zhang1, Xiaoying Wang1, Shuwei Wei1, Yue Zhao1, Qin Ding2, Yucui Han3, Lingjian Ma4. 1. College of Agronomy, Northwest A&F University, Yangling, 712100, China. 2. College of Horticulture, Northwest A&F University, Yangling, 712100, China. dingqin@nwsuaf.edu.cn. 3. College of Agronomy, Northwest A&F University, Yangling, 712100, China. 455205716@qq.com. 4. College of Agronomy, Northwest A&F University, Yangling, 712100, China. malingjian@nwsuaf.edu.cn.
Abstract
BACKGROUND: Heavy metal cadmium (Cd) is a common environmental pollutant in soils, which has an negative impacts on crop growth and development. At present, cadmium has become a major soil and water heavy metal pollutant, which not only causes permanent and irreversible health problems for humans, but also causes a significant reduction in crop yields. RESULTS: This study examined the chemical forms of Cd in the roots of two wheat varieties (M1019 and Xinong20) by continuous extraction and analyzed differences in distribution characteristics of Cd in the root cell wall, cytoplasm, and organelles by elemental content determination and subcellular separation. Furthermore, we conducted proteomics analysis of the roots of the two varieties under Cd pollution using mass spectrometry quantitative proteomics techniques. A total of 11,651 proteins were identified, of which 10,532 proteins contained quantitative information. In addition, the differentially expressed proteins in the two varieties were related to DNA replication and repair, protein metabolism, and the glutathione metabolism pathway. CONCLUSION: The results of this study improve our understanding of the mechanism of plant responses to Cd stress.
BACKGROUND:Heavy metal cadmium (Cd) is a common environmental pollutant in soils, which has an negative impacts on crop growth and development. At present, cadmium has become a major soil and water heavy metal pollutant, which not only causes permanent and irreversible health problems for humans, but also causes a significant reduction in crop yields. RESULTS: This study examined the chemical forms of Cd in the roots of two wheat varieties (M1019 and Xinong20) by continuous extraction and analyzed differences in distribution characteristics of Cd in the root cell wall, cytoplasm, and organelles by elemental content determination and subcellular separation. Furthermore, we conducted proteomics analysis of the roots of the two varieties under Cd pollution using mass spectrometry quantitative proteomics techniques. A total of 11,651 proteins were identified, of which 10,532 proteins contained quantitative information. In addition, the differentially expressed proteins in the two varieties were related to DNA replication and repair, protein metabolism, and the glutathione metabolism pathway. CONCLUSION: The results of this study improve our understanding of the mechanism of plant responses to Cdstress.
Entities:
Keywords:
Cd stress; Chemical forms; Proteomics analysis; Wheat roots
Authors: Harald Marx; Catherine E Minogue; Dhileepkumar Jayaraman; Alicia L Richards; Nicholas W Kwiecien; Alireza F Siahpirani; Shanmugam Rajasekar; Junko Maeda; Kevin Garcia; Angel R Del Valle-Echevarria; Jeremy D Volkening; Michael S Westphall; Sushmita Roy; Michael R Sussman; Jean-Michel Ané; Joshua J Coon Journal: Nat Biotechnol Date: 2016-10-17 Impact factor: 54.908