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Literature DB >> 32378811

Human Papillomavirus Quasivirus Production and Infection of Primary Human Keratinocytes.

Abstract

This protocol describes the production of human papillomavirus (HPV)-derived quasiviruses. Quasiviruses are infectious particles that are produced in 293TT packaging cells and contain a complete viral genome. We describe methods for infection of primary human keratinocytes with HPV quasiviruses, as well as assays to measure early viral DNA replication and transcription. Published 2020. U.S. Government. Basic Protocol 1: Transfection, harvest, and isolation of HPV quasiviruses Alternate Protocol 1: Packaging HPV DNA replicated in 293TT cells Alternate Protocol 2: Production of higher-purity quasivirus using the "Ripcord" method Support Protocol 1: Production of HPV minicircles Support Protocol 2: Production of recircularized HPV genomes Support Protocol 3: Screening of fractions for viral proteins Support Protocol 4: Screening of fractions for viral DNA Support Protocol 5: Measuring viral titer Support Protocol 6: Quantitation of quasivirions Basic Protocol 2: Infection of primary human foreskin keratinocytes with quasivirus Basic Protocol 3: HPV quasivirus transcription assay Basic Protocol 4: HPV quasivirus replication assay. Published 2020. This article is a US Government work and is in the public domain in the USA.

Entities: Chemical

Keywords: HPV; infection; keratinocyte; papillomavirus; quasivirus; virion

Mesh：

Substances：
Viral Proteins

Year: 2020 PMID： 32378811 PMCID： PMC7263449 DOI： 10.1002/cpmc.101

Source DB: PubMed Journal: Curr Protoc Microbiol ISSN： 1934-8525

14 in total

1. Differentiation of HPV-containing cells using organotypic "raft" culture or methylcellulose.

Authors: Regina Wilson; Laimonis A Laimins
Journal: Methods Mol Med Date: 2005

2. Nucleotide sequence and comparative analysis of the human papillomavirus type 18 genome. Phylogeny of papillomaviruses and repeated structure of the E6 and E7 gene products.

Authors: S T Cole; O Danos
Journal: J Mol Biol Date: 1987-02-20 Impact factor: 5.469

3. Generation of HPV pseudovirions using transfection and their use in neutralization assays.

Authors: Christopher B Buck; Diana V Pastrana; Douglas R Lowy; John T Schiller
Journal: Methods Mol Med Date: 2005

4. Analysis of Human Papillomavirus Genome Replication Using Two- and Three-Dimensional Agarose Gel Electrophoresis.

Authors: Liisi Henno; Eva-Maria Tombak; Jelizaveta Geimanen; Marit Orav; Ene Ustav; Mart Ustav
Journal: Curr Protoc Microbiol Date: 2017-05-16

5. Using organotypic (raft) epithelial tissue cultures for the biosynthesis and isolation of infectious human papillomaviruses.

Authors: Michelle A Ozbun; Nicole A Patterson
Journal: Curr Protoc Microbiol Date: 2014-08-01

6. Assembly of transfected DNA into chromatin: structural changes in the origin-promoter-enhancer region upon replication.

Authors: S Cereghini; M Yaniv
Journal: EMBO J Date: 1984-06 Impact factor: 11.598

7. A new type of papillomavirus DNA, its presence in genital cancer biopsies and in cell lines derived from cervical cancer.

Authors: M Boshart; L Gissmann; H Ikenberg; A Kleinheinz; W Scheurlen; H zur Hausen
Journal: EMBO J Date: 1984-05 Impact factor: 11.598

Human Papillomavirus Quasivirus Production and Infection of Primary Human Keratinocytes.

1. Differentiation of HPV-containing cells using organotypic "raft" culture or methylcellulose.

2. Nucleotide sequence and comparative analysis of the human papillomavirus type 18 genome. Phylogeny of papillomaviruses and repeated structure of the E6 and E7 gene products.

3. Generation of HPV pseudovirions using transfection and their use in neutralization assays.

4. Analysis of Human Papillomavirus Genome Replication Using Two- and Three-Dimensional Agarose Gel Electrophoresis.

5. Using organotypic (raft) epithelial tissue cultures for the biosynthesis and isolation of infectious human papillomaviruses.

6. Assembly of transfected DNA into chromatin: structural changes in the origin-promoter-enhancer region upon replication.

7. A new type of papillomavirus DNA, its presence in genital cancer biopsies and in cell lines derived from cervical cancer.

8. Sp100 provides intrinsic immunity against human papillomavirus infection.

9. Maturation of the human papillomavirus 16 capsid.

10. Biosafety: guidelines for working with pathogenic and infectious microorganisms.

1. Development of Keratinocyte Cell Lines Containing Extrachromosomal Human Papillomavirus Genomes.

Review 2. The long and winding road: human papillomavirus entry and subcellular trafficking.

3. The First Human Vulvar Intraepithelial Neoplasia Cell Line with Naturally Infected Episomal HPV18 Genome.

4. Histone Modifications in Papillomavirus Virion Minichromosomes.

5. Replication Kinetics for a Reporter Merkel Cell Polyomavirus.