Bacterial diversity in the intestinal mucosa of mice fed with Asparagus extract under high-fat diet condition.

The purpose of this study is to determine the effect of Asparagus on bacterial diversity in the intestinal mucosa of mice fed with high-fat diet, thus providing theoretical basis for the development and research of Asparagus products. Twelve healthy male Kunming mice and twelve healthy female Kunming mice were chosen and randomly divided into normal group, model group, Asparagus group, and lipid-lowering decoction group, with six mice in each group. After establishing the models of mice fed with high-fat diet through feeding with high-fat diet, the mice in the Asparagus group were gavaged with Asparagus juice, those in the lipid-lowering decoction group were gavaged with lipid-lowering decoction, and those in the normal group and high-fat diet group were gavaged with the equal amount of distilled water. Intestinal mucosa from the jejunum to ileum were collected, and DNA was extracted from each mice. The characteristics of the intestinal microbial species were analyzed by PacBio Sequel-based 16S rRNA sequencing. Result showed that the total OTU reached 1559 in the normal group, 1750 in the high-fat diet group, 1795 in the lipid-Lowering decoction group, and 1635 in the Asparagus group, which indicated that the Asparagus juice could inhibit the total OUT of intestinal bacteria. The analysis on sample community diversity indicated that the richness, diversity, richness estimation, and diversity in the Asparagus Group, lipid-lowering decoction group, and normal group were lower than those in the high-fat diet group. Bacteriophyta classification analysis indicated that the relative abundance of Firmicutes, Bacteroidetes, and Actinobacteria in the Asparagus group was between that in the high-fat diet group and normal group. In conclusion, Asparagus can affect the diversity of bacteria in the intestinal mucosa of mice fed with high-fat diet, and achieve a lipid-lowering effect by regulating the intestinal microecology of mice fed with high-fat diet. © King Abdulaziz City for Science and Technology 2020.