Wei Xia1,2, Degui Gong2, Xiaoping Qin2, Zhuo Cai3. 1. Department of Cell biology, Southern Medical University, Guangzhou 510515, China. 2. Department of Clinical Laboratory, 74th Army Hospital of PLA, Guangzhou 510310, China. 3. Department of Pharmacy, Air Force Hospital of Southern Theater Command of PLA, Guangzhou 510602, China.
Abstract
OBJECTIVE: To investigate the effects of miR-671-3p on the proliferation and invasion of breast cancer cells and explore the possible mechanism. METHODS: We examined the expressions of miR-671-3p in human normal epithelial cells (MCF-10A) and breast cancer cell lines (MCF-7, MDA-MB-231, and SK-BR3) using RT-PCR. The effects of transfection with a miR-671-3p mimic or inhibitor on the proliferation, migration and invasion of MCF-7 cells were evaluated using CCK-8 assay and Transwell chamber assay. The target gene of miR-671-3p was predicated with Targetscan and validated by a dual luciferase reporter system and Western blotting. RESULTS: The expression of miR-671-3p was significantly lower in breast cancer cells than in normal breast epithelial cells. Compared with negative control group, MCF-7 cells with miR-671-3p overexpression exhibited significantly reduced proliferation and invasion, whereas inhibition of miR-671-3p obviously promoted the cell proliferation and invasion. Luciferase reporter assay demonstrated that DEPTOR was the target gene of miR-671-3p, and miR-671-3p overexpression caused significant down-regulation of the protein expression of DEPTOR. CONCLUSIONS: MiR-671-3p suppresses the proliferation and invasion of breast cancer cell line MCF-7 by directly targeting DEPTOR protein.
OBJECTIVE: To investigate the effects of miR-671-3p on the proliferation and invasion of breast cancer cells and explore the possible mechanism. METHODS: We examined the expressions of miR-671-3p in human normal epithelial cells (MCF-10A) and breast cancer cell lines (MCF-7, MDA-MB-231, and SK-BR3) using RT-PCR. The effects of transfection with a miR-671-3p mimic or inhibitor on the proliferation, migration and invasion of MCF-7 cells were evaluated using CCK-8 assay and Transwell chamber assay. The target gene of miR-671-3p was predicated with Targetscan and validated by a dual luciferase reporter system and Western blotting. RESULTS: The expression of miR-671-3p was significantly lower in breast cancer cells than in normal breast epithelial cells. Compared with negative control group, MCF-7 cells with miR-671-3p overexpression exhibited significantly reduced proliferation and invasion, whereas inhibition of miR-671-3p obviously promoted the cell proliferation and invasion. Luciferase reporter assay demonstrated that DEPTOR was the target gene of miR-671-3p, and miR-671-3p overexpression caused significant down-regulation of the protein expression of DEPTOR. CONCLUSIONS:MiR-671-3p suppresses the proliferation and invasion of breast cancer cell line MCF-7 by directly targeting DEPTOR protein.
Entities:
Keywords:
DEPTOR; breast cancer; cell proliferation; miR-671-3p; neoplasm invasiveness
Authors: Timothy R Peterson; Mathieu Laplante; Carson C Thoreen; Yasemin Sancak; Seong A Kang; W Michael Kuehl; Nathanael S Gray; David M Sabatini Journal: Cell Date: 2009-05-14 Impact factor: 41.582
Authors: Hua Li; Grace Y Sun; Yongchao Zhao; Dafydd Thomas; Joel K Greenson; Mark M Zalupski; Edgar Ben-Josef; Yi Sun Journal: Oncotarget Date: 2014-12-30