Yuxi Huang1, Hao Zhang1, Shuang Zhang1, Jian Sun1. 1. Department of Nephrology and Rheumatology, Third Xiangya Hospital, Central South University, Changsha 410013, China.
Abstract
OBJECTIVE: To detect the expressions of vitamin D receptor (VDR) in peripheral blood monocytes (PBMCs) and its association with monocyte chemoattractant protein-1 (MCP-1) in patients with systemic lupus erythemaotsus (SLE). METHODS: We examined the expressions of VDR and MCP-1 mRNAs in the PBMCs in 60 SLE patients and 28 healthy individuals using real- time quantitative PCR. We also detected the expression of VDR protein in the PBMCs using Western blotting and peripheral blood MCP-1 level using ELISA for these participants. The correlation of VDR and MCP-1 expressions with the disease activity index of SLE (SLEDAI) of the patients were analyzed. RESULTS: The expressions of VDR mRNA and protein in the PBMCs were significantly lower in patients with SLE than in the healthy individuals (P < 0.01), and that in the patients with active disease was lower than in remission (P < 0.05). The MCP-1 mRNA expression in the PBMCs and its serum levels were significantly increased in SLE patients as compared with the healthy individuals (P < 0.01), and the increase was significantly more obvious in the patients with active disease than in those in remission (P < 0.01). Pearson correlation analysis showed that VDR mRNA in the PBMCs was negatively correlated with SLEDAI (r=-0.417, P=0.001); negative correlations were also found between VDR mRNA and MCP-1 mRNA(r=-0.554, P=0.000) and between VDR protein expression and serum MCP-1 level (r=-0.400, P=0.028). CONCLUSIONS: The down-regulation of VDR expression in the PBMC is negatively correlated with the disease activity of SLE. VDR may play an important role in the pathogenesis of SLE by affecting the expression of MCP-1.
OBJECTIVE: To detect the expressions of vitamin D receptor (VDR) in peripheral blood monocytes (PBMCs) and its association with monocyte chemoattractant protein-1 (MCP-1) in patients with systemic lupus erythemaotsus (SLE). METHODS: We examined the expressions of VDR and MCP-1 mRNAs in the PBMCs in 60 SLEpatients and 28 healthy individuals using real- time quantitative PCR. We also detected the expression of VDR protein in the PBMCs using Western blotting and peripheral blood MCP-1 level using ELISA for these participants. The correlation of VDR and MCP-1 expressions with the disease activity index of SLE (SLEDAI) of the patients were analyzed. RESULTS: The expressions of VDR mRNA and protein in the PBMCs were significantly lower in patients with SLE than in the healthy individuals (P < 0.01), and that in the patients with active disease was lower than in remission (P < 0.05). The MCP-1 mRNA expression in the PBMCs and its serum levels were significantly increased in SLEpatients as compared with the healthy individuals (P < 0.01), and the increase was significantly more obvious in the patients with active disease than in those in remission (P < 0.01). Pearson correlation analysis showed that VDR mRNA in the PBMCs was negatively correlated with SLEDAI (r=-0.417, P=0.001); negative correlations were also found between VDR mRNA and MCP-1 mRNA(r=-0.554, P=0.000) and between VDR protein expression and serum MCP-1 level (r=-0.400, P=0.028). CONCLUSIONS: The down-regulation of VDR expression in the PBMC is negatively correlated with the disease activity of SLE. VDR may play an important role in the pathogenesis of SLE by affecting the expression of MCP-1.
Authors: Maria-Dolores Sanchez-Niño; Milica Bozic; Elizabeth Córdoba-Lanús; Petya Valcheva; Olga Gracia; Merce Ibarz; Elvira Fernandez; Juan F Navarro-Gonzalez; Alberto Ortiz; Jose Manuel Valdivielso Journal: Am J Physiol Renal Physiol Date: 2011-12-14