Literature DB >> 32376546

[Laboratory testing techniques for SARS-CoV-2].

Feng Qiu1, Huijun Wang1, Zikang Zhang1, Longbin Cao1, Chenlong Wang2, Jingbiao Wu3, Qingfeng Du2.   

Abstract

The ongoing epidemic of caused by the coronavirus SARS-CoV-2 starting in December 2019 poses a serious public health threat globally. The virus is highly infectious and transmitted mainly through droplets and contacts, and is associated with a high risk of pneumonia. A small number of patients may present with acute respiratory distress syndrome with severe respiratory complications, which can lead even to death. The selection of appropriate detection techniques and methods for accurate and rapid identification of pathogens therefore plays a key role in improving the diagnosis and treatment of the patients and containing the outbreak. In this review, the authors gives an overview of the virus laboratory detection technology, including virus isolation and culture, real-time fluorescent PCR, gene sequencing, serological antibody detection, and the gene editing technology based on CRISPR/Cas13 system. These techniques are expected to provide valuable assistance in controlling the epidemic and new ideas for future researches.

Entities:  

Keywords:  COVID-19; PCR; SARS-CoV-2; gene editing

Mesh:

Substances:

Year:  2020        PMID: 32376546      PMCID: PMC7086127          DOI: 10.12122/j.issn.1673-4254.2020.02.04

Source DB:  PubMed          Journal:  Nan Fang Yi Ke Da Xue Xue Bao        ISSN: 1673-4254


  18 in total

1.  Nucleic acid detection with CRISPR-Cas13a/C2c2.

Authors:  Jonathan S Gootenberg; Omar O Abudayyeh; Jeong Wook Lee; Patrick Essletzbichler; Aaron J Dy; Julia Joung; Vanessa Verdine; Nina Donghia; Nichole M Daringer; Catherine A Freije; Cameron Myhrvold; Roby P Bhattacharyya; Jonathan Livny; Aviv Regev; Eugene V Koonin; Deborah T Hung; Pardis C Sabeti; James J Collins; Feng Zhang
Journal:  Science       Date:  2017-04-13       Impact factor: 47.728

2.  Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6.

Authors:  Jonathan S Gootenberg; Omar O Abudayyeh; Max J Kellner; Julia Joung; James J Collins; Feng Zhang
Journal:  Science       Date:  2018-02-15       Impact factor: 47.728

3.  Delivery Aspects of CRISPR/Cas for in Vivo Genome Editing.

Authors:  Danny Wilbie; Johanna Walther; Enrico Mastrobattista
Journal:  Acc Chem Res       Date:  2019-05-17       Impact factor: 22.384

4.  RNA based mNGS approach identifies a novel human coronavirus from two individual pneumonia cases in 2019 Wuhan outbreak.

Authors:  Liangjun Chen; Weiyong Liu; Qi Zhang; Ke Xu; Guangming Ye; Weichen Wu; Ziyong Sun; Fang Liu; Kailang Wu; Bo Zhong; Yi Mei; Wenxia Zhang; Yu Chen; Yirong Li; Mang Shi; Ke Lan; Yingle Liu
Journal:  Emerg Microbes Infect       Date:  2020-02-05       Impact factor: 7.163

5.  RNA sequencing by direct tagmentation of RNA/DNA hybrids.

Authors:  Lin Di; Yusi Fu; Yue Sun; Jie Li; Lu Liu; Jiacheng Yao; Guanbo Wang; Yalei Wu; Kaiqin Lao; Raymond W Lee; Genhua Zheng; Jun Xu; Juntaek Oh; Dong Wang; X Sunney Xie; Yanyi Huang; Jianbin Wang
Journal:  Proc Natl Acad Sci U S A       Date:  2020-01-27       Impact factor: 11.205

6.  Genomic characterisation and epidemiology of 2019 novel coronavirus: implications for virus origins and receptor binding.

Authors:  Roujian Lu; Xiang Zhao; Juan Li; Peihua Niu; Bo Yang; Honglong Wu; Wenling Wang; Hao Song; Baoying Huang; Na Zhu; Yuhai Bi; Xuejun Ma; Faxian Zhan; Liang Wang; Tao Hu; Hong Zhou; Zhenhong Hu; Weimin Zhou; Li Zhao; Jing Chen; Yao Meng; Ji Wang; Yang Lin; Jianying Yuan; Zhihao Xie; Jinmin Ma; William J Liu; Dayan Wang; Wenbo Xu; Edward C Holmes; George F Gao; Guizhen Wu; Weijun Chen; Weifeng Shi; Wenjie Tan
Journal:  Lancet       Date:  2020-01-30       Impact factor: 79.321

7.  Full-genome evolutionary analysis of the novel corona virus (2019-nCoV) rejects the hypothesis of emergence as a result of a recent recombination event.

Authors:  D Paraskevis; E G Kostaki; G Magiorkinis; G Panayiotakopoulos; G Sourvinos; S Tsiodras
Journal:  Infect Genet Evol       Date:  2020-01-29       Impact factor: 3.342

8.  Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR.

Authors:  Victor M Corman; Olfert Landt; Marco Kaiser; Richard Molenkamp; Adam Meijer; Daniel Kw Chu; Tobias Bleicker; Sebastian Brünink; Julia Schneider; Marie Luisa Schmidt; Daphne Gjc Mulders; Bart L Haagmans; Bas van der Veer; Sharon van den Brink; Lisa Wijsman; Gabriel Goderski; Jean-Louis Romette; Joanna Ellis; Maria Zambon; Malik Peiris; Herman Goossens; Chantal Reusken; Marion Pg Koopmans; Christian Drosten
Journal:  Euro Surveill       Date:  2020-01

9.  A Novel Coronavirus from Patients with Pneumonia in China, 2019.

Authors:  Na Zhu; Dingyu Zhang; Wenling Wang; Xingwang Li; Bo Yang; Jingdong Song; Xiang Zhao; Baoying Huang; Weifeng Shi; Roujian Lu; Peihua Niu; Faxian Zhan; Xuejun Ma; Dayan Wang; Wenbo Xu; Guizhen Wu; George F Gao; Wenjie Tan
Journal:  N Engl J Med       Date:  2020-01-24       Impact factor: 91.245

Review 10.  Measures for diagnosing and treating infections by a novel coronavirus responsible for a pneumonia outbreak originating in Wuhan, China.

Authors:  Fei Yu; Lanying Du; David M Ojcius; Chungen Pan; Shibo Jiang
Journal:  Microbes Infect       Date:  2020-02-01       Impact factor: 2.700

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  7 in total

Review 1.  Immunological perspectives on the pathogenesis, diagnosis, prevention and treatment of COVID-19.

Authors:  Yanghong Ni; Aqu Alu; Hong Lei; Yang Wang; Min Wu; Xiawei Wei
Journal:  Mol Biomed       Date:  2021-01-20

Review 2.  SARS-CoV-2 Molecular Diagnostics in China.

Authors:  Yanjun Lu; Ziyong Sun
Journal:  Clin Lab Med       Date:  2022-03-04       Impact factor: 2.172

3.  COVID-19 suspicion revealed to be fat embolism syndrome.

Authors:  Salim Mazouz; Ouissal Aissaoui; Mohamed Anass Fehdi; Afak Nsiri; Rachid AlHarrar
Journal:  Pan Afr Med J       Date:  2020-06-17

Review 4.  Current methods and prospects of coronavirus detection.

Authors:  Jiaqi Bu; Zhiwei Deng; Hui Liu; Jiacheng Li; Yanjing Yang; Shian Zhong
Journal:  Talanta       Date:  2020-12-31       Impact factor: 6.556

Review 5.  Immunological perspectives on the pathogenesis, diagnosis, prevention and treatment of COVID-19.

Authors:  Yanghong Ni; Aqu Alu; Hong Lei; Yang Wang; Min Wu; Xiawei Wei
Journal:  Mol Biomed       Date:  2021-01-20

6.  Preliminary Analysis of B- and T-Cell Responses to SARS-CoV-2.

Authors:  Li-Xia Zhang; Shu-Yan Miao; Zhong-Hua Qin; Jun-Pin Wu; Huai-Yong Chen; Hai-Bai Sun; Yi Xie; Yan-Qing Du; Jun Shen
Journal:  Mol Diagn Ther       Date:  2020-10       Impact factor: 4.476

Review 7.  Laboratory diagnosis of COVID-19 in China: A review of challenging cases and analysis.

Authors:  Ran Jing; Timothy Kudinha; Meng-Lan Zhou; Meng Xiao; He Wang; Wen-Hang Yang; Ying-Chun Xu; Po-Ren Hsueh
Journal:  J Microbiol Immunol Infect       Date:  2020-10-17       Impact factor: 4.399

  7 in total

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