Literature DB >> 3237544

Analysis of human pituitary tumors by in situ hybridization.

R V Lloyd1.   

Abstract

A procedure for performing in situ hybridization histochemistry (ISH) on frozen and paraffin sections of human pituitary tissues is described. The use of oligonucleotide probes for hPRL and hGH labeled with 35S allowed detection of a specific messenger RNA in frozen and paraffin sections. This technique can be combined with immunochemistry to localize both the gene product and the hormone(s) produced by specific cells and should be very helpful in the characterization of normal and neoplastic human pituitary cells.

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Year:  1988        PMID： 3237544     DOI： 10.1016/S0344-0338(88)80009-8

Source DB:  PubMed          Journal:  Pathol Res Pract        ISSN： 0344-0338            Impact factor:   3.250


  7 in total

1.  Combined non-isotopic in situ hybridisation and indirect immunohistochemical analysis of hormone production in the rat pituitary gland.

Authors:  N Sanno; A Matsuno; J Itoh; K Kakimoto; A Teramoto; R Y Osamura
Journal:  Clin Mol Pathol       Date:  1996-02

Review 2.  Human pituitary adenomas. Recent advances in morphological studies.

Authors:  G Giannattasio; M Bassetti
Journal:  J Endocrinol Invest       Date:  1990-05       Impact factor: 4.256

3.  Application of biotinylated oligonucleotide probes to the detection of pituitary hormone mRNA using northern blot analysis, in situ hybridization at the light- and electron-microscope levels.

Authors:  A Matsuno; A Teramoto; S Takekoshi; H Utsunomiya; Y Ohsugi; S Kishikawa; R Y Osamura; T Kirino; R V Lloyd
Journal:  Histochem J       Date:  1994-10

4.  GH-, PRL-, POMC-, beta-TSH-, beta-LH-, beta-FSH-mRNA in gonadotroph adenomas of the pituitary by in situ hybridization in comparison with immunostaining and clinical data.

Authors:  A Münscher; M Schmid; W Saeger; S Schreiber; D K Lüdecke
Journal:  Endocr Pathol       Date:  2001       Impact factor: 3.943

Review 5.  Molecular determinants of pituitary cytodifferentiation.

Authors:  S L Asa; S Ezzat
Journal:  Pituitary       Date:  1999-05       Impact factor: 4.107

6.  HGH, PRL and beta HCG/beta LH gene expression in clinically inactive pituitary adenomas detected by in situ hybridization.

Authors:  E Bäz; W Saeger; H Uhlig; S Fehr; D K Lüdecke
Journal:  Virchows Arch A Pathol Anat Histopathol       Date:  1991

7.  HGH, PRL, and ACTH Gene Expression in Clinically Nonfunctioning Adenomas Detected with Nonisotopic In Situ Hybridization Method.

Authors:  Akira Matsuno; Akira Teramoto; Susumu Takekoshi; Naoko Sanno; R. Yoshiyuki Osamura; Takaaki Kirino
Journal:  Endocr Pathol       Date:  1995       Impact factor: 3.943
  7 in total

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