Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Combining native and 'omics' mass spectrometry to identify endogenous ligands bound to membrane proteins.

Literature DB >> 32371966

Combining native and 'omics' mass spectrometry to identify endogenous ligands bound to membrane proteins.

Joseph Gault¹, Idlir Liko^2,3, Michael Landreh⁴, Denis Shutin², Jani Reddy Bolla², Damien Jefferies⁵, Mark Agasid², Hsin-Yung Yen³, Marcus J G W Ladds⁴, David P Lane⁴, Syma Khalid⁵, Christopher Mullen⁶, Philip M Remes⁶, Romain Huguet⁶, Graeme McAlister⁶, Michael Goodwin⁶, Rosa Viner⁶, John E P Syka⁶, Carol V Robinson⁷.

Abstract

Ligands bound to protein assemblies provide critical information for function, yet are often difficult to capture and define. Here we develop a top-down method, 'nativeomics', unifying 'omics' (lipidomics, proteomics, metabolomics) analysis with native mass spectrometry to identify ligands bound to membrane protein assemblies. By maintaining the link between proteins and ligands, we define the lipidome/metabolome in contact with membrane porins and a mitochondrial translocator to discover potential regulators of protein function.

Entities: Chemical

Mesh：

Substances：

Year: 2020 PMID： 32371966 PMCID： PMC7332344 DOI： 10.1038/s41592-020-0821-0

Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547

39 in total

1. Novel parallelized quadrupole/linear ion trap/Orbitrap tribrid mass spectrometer improving proteome coverage and peptide identification rates.

Authors: Michael W Senko; Philip M Remes; Jesse D Canterbury; Raman Mathur; Qingyu Song; Shannon M Eliuk; Chris Mullen; Lee Earley; Mark Hardman; Justin D Blethrow; Huy Bui; August Specht; Oliver Lange; Eduard Denisov; Alexander Makarov; Stevan Horning; Vlad Zabrouskov
Journal: Anal Chem Date: 2013-11-27 Impact factor: 6.986

2. Protein structure. Crystal structures of translocator protein (TSPO) and mutant mimic of a human polymorphism.

Authors: Fei Li; Jian Liu; Yi Zheng; R Michael Garavito; Shelagh Ferguson-Miller
Journal: Science Date: 2015-01-30 Impact factor: 47.728

Review 3. The G protein-coupled receptors deorphanization landscape.

Authors: Céline Laschet; Nadine Dupuis; Julien Hanson
Journal: Biochem Pharmacol Date: 2018-02-15 Impact factor: 5.858

4. Triple-Stage Mass Spectrometry Unravels the Heterogeneity of an Endogenous Protein Complex.

Authors: Gili Ben-Nissan; Mikhail E Belov; David Morgenstern; Yishai Levin; Orly Dym; Galina Arkind; Carni Lipson; Alexander A Makarov; Michal Sharon
Journal: Anal Chem Date: 2017-04-06 Impact factor: 6.986

5. Shorthand notation for lipid structures derived from mass spectrometry.

Authors: Gerhard Liebisch; Juan Antonio Vizcaíno; Harald Köfeler; Martin Trötzmüller; William J Griffiths; Gerd Schmitz; Friedrich Spener; Michael J O Wakelam
Journal: J Lipid Res Date: 2013-04-02 Impact factor: 5.922

6. Membrane curvature allosterically regulates the phosphatidylinositol cycle, controlling its rate and acyl-chain composition of its lipid intermediates.

Authors: José Carlos Bozelli; William Jennings; Stephanie Black; Yu Heng Hou; Darius Lameire; Preet Chatha; Tomohiro Kimura; Bob Berno; Adree Khondker; Maikel C Rheinstädter; Richard M Epand
Journal: J Biol Chem Date: 2018-09-20 Impact factor: 5.486

7. Top-down characterization of endogenous protein complexes with native proteomics.

Authors: Owen S Skinner; Nicole A Haverland; Luca Fornelli; Rafael D Melani; Luis H F Do Vale; Henrique S Seckler; Peter F Doubleday; Luis F Schachner; Kristina Srzentić; Neil L Kelleher; Philip D Compton
Journal: Nat Chem Biol Date: 2017-11-13 Impact factor: 15.040

8. Structural basis of nucleotide sugar transport across the Golgi membrane.

Authors: Joanne L Parker; Simon Newstead
Journal: Nature Date: 2017-11-15 Impact factor: 49.962

9. Lipid binding attenuates channel closure of the outer membrane protein OmpF.

Authors: Idlir Liko; Matteo T Degiacomi; Sejeong Lee; Thomas D Newport; Joseph Gault; Eamonn Reading; Jonathan T S Hopper; Nicholas G Housden; Paul White; Matthew Colledge; Altin Sula; B A Wallace; Colin Kleanthous; Phillip J Stansfeld; Hagan Bayley; Justin L P Benesch; Timothy M Allison; Carol V Robinson
Journal: Proc Natl Acad Sci U S A Date: 2018-06-11 Impact factor: 11.205

10. Discovery of Human Signaling Systems: Pairing Peptides to G Protein-Coupled Receptors.

Authors: Simon R Foster; Alexander S Hauser; Line Vedel; Ryan T Strachan; Xi-Ping Huang; Ariana C Gavin; Sushrut D Shah; Ajay P Nayak; Linda M Haugaard-Kedström; Raymond B Penn; Bryan L Roth; Hans Bräuner-Osborne; David E Gloriam
Journal: Cell Date: 2019-10-31 Impact factor: 41.582

30 in total

1. Native Ambient Mass Spectrometry Enables Analysis of Intact Endogenous Protein Assemblies up to 145 kDa Directly from Tissue.

Authors: Oliver J Hale; James W Hughes; Emma K Sisley; Helen J Cooper
Journal: Anal Chem Date: 2022-03-31 Impact factor: 6.986

Review 2. High-Resolution Native Mass Spectrometry.

Authors: Sem Tamara; Maurits A den Boer; Albert J R Heck
Journal: Chem Rev Date: 2021-08-20 Impact factor: 72.087

3. Uniting Native Capillary Electrophoresis and Multistage Ultraviolet Photodissociation Mass Spectrometry for Online Separation and Characterization of Escherichia coli Ribosomal Proteins and Protein Complexes.

Authors: M Rachel Mehaffey; Qiangwei Xia; Jennifer S Brodbelt
Journal: Anal Chem Date: 2020-11-06 Impact factor: 6.986

Combining native and 'omics' mass spectrometry to identify endogenous ligands bound to membrane proteins.

1. Novel parallelized quadrupole/linear ion trap/Orbitrap tribrid mass spectrometer improving proteome coverage and peptide identification rates.

2. Protein structure. Crystal structures of translocator protein (TSPO) and mutant mimic of a human polymorphism.

Review 3. The G protein-coupled receptors deorphanization landscape.

4. Triple-Stage Mass Spectrometry Unravels the Heterogeneity of an Endogenous Protein Complex.

5. Shorthand notation for lipid structures derived from mass spectrometry.

6. Membrane curvature allosterically regulates the phosphatidylinositol cycle, controlling its rate and acyl-chain composition of its lipid intermediates.

7. Top-down characterization of endogenous protein complexes with native proteomics.

8. Structural basis of nucleotide sugar transport across the Golgi membrane.

9. Lipid binding attenuates channel closure of the outer membrane protein OmpF.

10. Discovery of Human Signaling Systems: Pairing Peptides to G Protein-Coupled Receptors.

1. Native Ambient Mass Spectrometry Enables Analysis of Intact Endogenous Protein Assemblies up to 145 kDa Directly from Tissue.

Review 2. High-Resolution Native Mass Spectrometry.

3. Uniting Native Capillary Electrophoresis and Multistage Ultraviolet Photodissociation Mass Spectrometry for Online Separation and Characterization of Escherichia coli Ribosomal Proteins and Protein Complexes.

4. Top-down proteomics: challenges, innovations, and applications in basic and clinical research.

5. Tandem surface-induced dissociation of protein complexes on an ultrahigh resolution platform.

Review 6. Novel Strategies to Address the Challenges in Top-Down Proteomics.

Review 7. Native Mass Spectrometry of Membrane Proteins.

Review 8. Label-free visual proteomics: Coupling MS- and EM-based approaches in structural biology.

Review 9. Approaches to Heterogeneity in Native Mass Spectrometry.

Review 10. Higher-order structural characterisation of native proteins and complexes by top-down mass spectrometry.