Alicia Lacoma1,2, Laura Usón3,4, Gracia Mendoza3,4, Victor Sebastián3,4, Esther Garcia-Garcia1, Beatriz Muriel-Moreno1, Jose Domínguez1,2, Manuel Arruebo3,4, Cristina Prat1,2,5. 1. Microbiology Department, Hospital Universitari Germans Trias i Pujol, Fundació Institut d'Investigació en Ciències de la Salut GermansTrias i Pujol, Universitat Autònoma de Barcelona, Badalona, Spain. 2. CIBER Enfermedades Respiratorias (CIBERES), Instituto de Salud Carlos III, Spain. 3. Institute of Nanoscience of Aragon (INA), Department of Chemical Engineering & Environmental Technologies, University of Zaragoza & Instituto de Ciencia de Materiales de Aragón (ICMA), Universidad de Zaragoza-CSIC, 50018, Zaragoza, Spain. 4. CIBER Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Instituto de Salud Carlos III, Spain. 5. Julius Centre for Health Sciences and Primary Care, University Medical Center Utrecht, Utrecht University, Utrecht, The Netherlands.
Abstract
Aim: First, to compare in vitro minimum inhibitory concentrations (MIC) of free cloxacillin and cloxacillin-containing nanoparticles (NP) against methicillin-susceptible (MSSA) and resistant Staphylococcus aureus (MRSA) and second, to assess NP antimicrobial activity against intracellular S. aureus. Methods: Poly(d,l-lactide-co-glycolide) acid (PLGA)-NP were loaded with cloxacillin and physico-chemically characterized. MICs were determined for reference strains Newman-(MSSA) and USA300-(MRSA). Murine alveolar macrophages were infected, and bacterial intracellular survival was assessed after incubating with free-cloxacillin or PLGA-cloxacillin-NP. Results & conclusion: For both isolates, MICs for antibiotic-loaded-NP were lower than those obtained with free cloxacillin, indicating that the drug encapsulation improves antimicrobial activity. A sustained antibiotic release was demonstrated when using the PLGA-cloxacillin-NP. When considering the lowest concentrations, the use of drug-loaded NP enabled a higher reduction of intracellular bacterial load.
Aim: First, to compare in vitro minimum inhibitory concentrations (MIC) of free cloxacillin and cloxacillin-containing nanoparticles (NP) against methicillin-susceptible (MSSA) and resistant Staphylococcus aureus (MRSA) and second, to assess NP antimicrobial activity against intracellular S. aureus. Methods:Poly(d,l-lactide-co-glycolide) acid (PLGA)-NP were loaded with cloxacillin and physico-chemically characterized. MICs were determined for reference strains Newman-(MSSA) and USA300-(MRSA). Murine alveolar macrophages were infected, and bacterial intracellular survival was assessed after incubating with free-cloxacillin or PLGA-cloxacillin-NP. Results & conclusion: For both isolates, MICs for antibiotic-loaded-NP were lower than those obtained with free cloxacillin, indicating that the drug encapsulation improves antimicrobial activity. A sustained antibiotic release was demonstrated when using the PLGA-cloxacillin-NP. When considering the lowest concentrations, the use of drug-loaded NP enabled a higher reduction of intracellular bacterial load.
Entities:
Keywords:
Staphylococcus aureus ; antimicrobial; drug delivery; intracellular infection; nanoparticle
Authors: Borel Ndezo Bisso; Christian Ramsès Tokam Kuaté; Nathalie Boulens; Eric Allémann; Florence Delie; Jean Paul Dzoyem Journal: Evid Based Complement Alternat Med Date: 2022-07-21 Impact factor: 2.650
Authors: Seyed Hesamoddin Bidooki; Teresa Alejo; Javier Sánchez-Marco; Roberto Martínez-Beamonte; Roubi Abuobeid; Juan Carlos Burillo; Roberto Lasheras; Victor Sebastian; María J Rodríguez-Yoldi; Manuel Arruebo; Jesús Osada Journal: Antioxidants (Basel) Date: 2022-03-18