Position of Orifice of Distobuccal Canal of Maxillary Second Molars Using Cone-Beam Computed Tomography.

BACKGROUND AND OBJECTIVES: This study aimed to assess the position of distobuccal canal orifice of maxillary second molars using cone-beam computed tomography (CBCT).
MATERIALS AND METHODS: A total of 301 CBCT scans of patients were retrieved from the archives of a radiology clinic, and the distance between the orifices of distobuccal and mesiobuccal root canals, the distance between the orifices of distobuccal and palatal root canals and the angle between the orifices of mesiobuccal, distobuccal and palatal root canals in the axial plane were measured on CBCT scans.
RESULTS: Of 301 maxillary second molars evaluated in this study, 202 (67.1%) had three canals and 99 (32.9%) had four canals. The distance between the distobuccal and mesiobuccal canal orifices (DM) ranged from 1.10 to 4.40 mm (mean: 2.1831±0.6370 mm). The distance between the distobuccal and palatal canal orifices (DP) ranged from 1.20 to 6.20 mm (mean: 3.8704±0.84944 mm). The mean angle between the mesiobuccal, distobuccal and palatal orifices (MDP) was 106.37±12.12075°.
CONCLUSION: Around one-third of maxillary second molars had four canals; the rest had three canals. No maxillary second molar with two canals was found. The orifice of distobuccal canal was located at 2 mm distance from the mesiobuccal canal orifice in distopalatal direction in the majority of cases. It had 3 to 4 mm distance from the palatal canal orifice in most cases. CBCT is recommended to find the orifice of distobuccal canal in cases in whom negotiation of this canal is challenging.

Introduction

Missing a root canal and inefficient cleaning and shaping of all root canals are among the most common causes of failure of non-surgical endodontic treatment. Many studies have evaluated the root canal morphology in different populations. However, this topic is highly important for endodontists and general dental clinicians.1,2 The maxillary first and second molars commonly require endodontic treatment. However, they have anatomical complexities in their root canal system.3,4 Several methods have been suggested for assessment of root canal morphology.2 Three-dimensional (3D) computed tomography (CT) is a relatively new modality and chief method for assessment of root canal morphology.5,6 Cone-beam computed tomography (CBCT) provides 3D scans of the maxillofacial skeleton and the teeth with a considerably lower patient radiation dose compared to the conventional CT. CBCT provides images of an object in axial, sagittal and coronal planes, which are used in 3D image reconstruction to determine the morphology and some other properties of an object. Moreover, CBCT is non-invasive and the samples remain intact after tomography.5,7 Evidence shows that dental clinicians can obtain valuable information about the root canal anatomy and morphology using CBCT.8,9 Lee et al10 used CBCT to assess the anatomy of the mesiobuccal canal of maxillary first and second molars in a Korean population. They detected a second mesiobuccal canal in 71.8% of first molars and 42.2% of second molars.

Alavi et al11 evaluated the root and canal morphology of maxillary molars in a Thai population. They observed that more than half of the mesiobuccal canals of first and second molars had two canals. Inter-canal communication was noted in 16% of the cases. Considering the variations in canal anatomy and morphology in different populations, this study aimed to assess the position of distobuccal canal orifice of maxillary second molars in a population residing in south-east Iran to racially determine the root canal orifice variations.

Materials and Methods

Patient Selection

This descriptive cross-sectional study was approved by the ethics committee of Zahedan University of Medical Sciences, Zahedan, Iran (IR.ZAUMS.REC. 1394.1498), and written informed consent was obtained from patients. CBCT scans were retrieved from the archives of radiology clinics in Zahedan city, Iran from 2016 to 2018. The CBCT scans had been obtained for preoperative assessment for implant treatment or for diagnostic purposes. A total of 301 three-rooted maxillary second molars of 273 patients (98 males and 175 females) with a mean age of 37.5 years (18 to 60 years) were evaluated. The inclusion criteria were as follows: The maxillary second molars had mature apices with no carious lesions and no defects. They had no coronal or root restoration, no previous endodontic treatment and no apical lesion. They did not have any calcification or root resorption. The CBCT scans had optimal quality.

CBCT

The CBCT scans had been taken using a CBCT scanner (Vatech Co., Ltd., Gyeonggi-do, Korea) with the exposure settings of 89 kVp, 5.4 mA, 6 s exposure time, 0.2 mm voxel size, 90x120 mm field of view, vertical position at the center and 1.5 mm slice thickness. All CBCT scans were evaluated by an experienced radiologist using Image Viewing Program (EZ3D Plus, Korea) in a dark room on a 14-inch LED flat-screen monitor (Sony) with 1280x800-pixel resolution.

Image Assessment

A total of 301 CBCT scans of maxillary second molars were evaluated. Number of roots and canals in each tooth, the distance between the center of distobuccal and mesiobuccal canal orifices, the distance between the center of distobuccal and palatal canal orifices and the angle between the mesiobuccal, distobuccal and palatal canal orifices (MDP) were all measured. Each image was evaluated in axial section at the level of canal orifice. All measurements and analyses were carried out by one endodontist and one radiologist twice with a two-week interval. In case of disagreement, another experienced endodontist evaluated the images to reach a consensus. The data were then obtained after the final calibration.

Results

The maxillary second molars had three canals in 67.1% and four canals in 32.9% of the cases. No case had two or more than four canals (Table 1).

Table 1

Number and Percentage of Root Canals of Maxillary Second Molars

Root Canal Number	2	3	4	5	Total
Frequency	0	202	99	0	301
Percentage	0.0	67.1	32.9	0.0	100.0

In 301 maxillary second molars with three and four canals, the distance between the distobuccal and mesiobuccal canal orifices (DM) ranged from 1.10 to 4.40 mm with a mean of 2.1831±0.63709 mm. The distance between the orifice of distobuccal and palatal canals (DP) ranged from 1.20 to 6.20 mm with a mean of 3.8704±0.84944 mm. The mean angle between the mesiobuccal, distobuccal and palatal orifices (MDP) was 106.37±12.12075° (Table 2).

Table 2

Mean, Standard Deviation, Minimum and Maximum Values of DM, DP in Millimeters and MDP in Degrees in Maxillary Second Molars (n=301)

Distance	Minimum(mm)	Maximum(mm)	Mean(mm)	Std. Deviation(mm)
DM	1.10	4.40	2.1831	0.63709
DP	1.20	6.20	3.8704	0.84944
MDP	80.40	144.50	106.37	12.12075

DM was in the range of 1.5 to 3 mm in 90% of the teeth; in the remaining 10%, it was in the range of 3.5 to 4.5 mm (Table 3). DP ranged from 3.5 to 5 mm in 80.4% of the cases. In 13.3% of the cases, it was in the range of 1.5 to 3 mm and in 6.3% of the cases, it was in the range of 5.5 to 6.5 mm (Table 4).

Table 3

DM (Distance Between the Distobuccal and Mesiobuccal Canal Orifices) Variations in Millimeters

DM	~1	~1.5	~2	~2.5	~3	~3.5	~4	~4.5	Total
Frequency	0	50	92	80	49	20	8	2	301
Percentage	0.0	16.6	30.6	26.6	16.3	6.6	2.7	0.7	100

Table 4

DP (Distance Between the Distobuccal and Palatal Canal Orifices) Variations in Millimeters

DP	~1	~1.5	~2	~2.5	~3	~3.5	~4	~4.5	~5	~5.5	~6	~6.5	Total
Frequency	0	3	6	13	18	56	81	53	52	11	7	1	301
Percentage	0.0	1	2	4.3	6	18.6	26.9	17.6	17.3	3.7	2.3	0.3	100

MDP was in the range of 80.4° to 144.5° such that this value ranged from 90 to 120° in 80% of the teeth. In 7.3%, this value was smaller than 90° and in 13.3%, it was larger than 120° (Tables 5 and 6 and Figure 1).

Table 5

Frequency Distribution of MDP Angle Variations (<90°, 90–120° and >120°)

MDP	Frequency	Percentage
<90°	22	7.3
90–120°	239	79.4
>120°	40	13.3
Total	301	100.0

Table 6

MDP (the Angle Formed by the Mesiobuccal, Distobuccal and Palatal Canal Orifices) Variability

MDP	80–90°	~100°	~110°	~120°	~130°	~140°	~150°	Total
Frequency	22	71	99	69	31	7	2	301
Percentage	7.3	23.6	32.9	22.9	10.3	2.3	0.7	100

Figure 1

Images of transverse section of the orifice in different cases. From left to right and from top to bottom: MDP<90° and MDP>140°. Blue values show distance between the orifices of distobuccal and mesiobuccal canals (DM), and distance between the orifices of distobuccal and palatal canals (DP). Green values show the angle between the orifices of mesiobuccal, distobuccal and palatal canals (MDP). Red values indicate the canal orifices of four-canal maxillary second molars.

Discussion

Success of root canal treatment depends on possession of a complete knowledge about tooth anatomy and morphology of the root canal system, which may be variable within the normal range.12 Although the external morphology and internal anatomy of the teeth appear to be the same, a high variability exists in this respect. Permanent teeth have wide variations in the number of roots, number and shape of canals in each root and root fusion.13 Several factors are responsible for the variability in results such as age, gender and race. Also, difference in the reported results may be due to study design and methodology.14,15

CBCT is a commonly used radiographic modality for assessment of the anatomy and morphology of the root canal system.16 It does not have the limitations of 2D radiography and has lower patient radiation dose and higher resolution than medical CT. CBCT is extensively used in implantology, orthodontics, oral and maxillofacial reconstruction, diagnosis prior to root canal treatment and assessment of the quality of root canal preparation, obturation and retreatment.17

Blattner et al, in 2010 showed that the second mesiobuccal canal could be detected in 78.95% of the cases and there was no significant difference in the ability of CBCT for detection of second mesiobuccal canal compared to clinical sectioning (gold standard).18 Angelopoulos et al, in 2008 indicated that CBCT provides reliable results since it does not cause magnification or distortion.19

Since studies on the position of distobuccal canal orifice of maxillary second molars are limited, this study used CBCT as a non-invasive modality to assess the position of distobuccal canal orifice of maxillary second molars. The current results showed that 67.1% of maxillary second molars had three canals and 32.9% had four canals.

Rouhani et al, in 2014 demonstrated that only 1.6% of maxillary second molars of an Iranian subpopulation had four roots and 98.4% had three roots.20 Zhang et al, in 2011 showed that maxillary second molars had one root in 10%, two roots in 8% and three roots in 81% of the cases.21 Kim et al, in 2012 demonstrated that maxillary second molars in a Korean population had three or four roots in 82.2% of the cases; an additional canal was present in distobuccal root in 0.30% of the cases.22

In this study, the mean distance between the orifices of distobuccal and mesiobuccal root canals was 2.18±0.63 mm (range 1.1 to 4.4 mm). This distance was in the range of 1.5 to 3 mm in 90% of the cases, which was in agreement with the results of Han et al, in 2012. They reported that this distance was 0.7 to 4.8 mm in a Chinese population and 1.5 to 3 mm in 81.39% of the cases.23 The current findings revealed that the mean distance between the distobuccal and palatal root canal orifices was 3.87±0.84 mm; this value ranged from 1.7 to 6 mm. In 80.4% of the samples, this distance ranged from 3.5 to 5 mm while it was <3 mm in 13.3% and >5 mm in 6.3%. In our study, this distance was in the range of 3 to 5 mm in 90% of the cases; this finding was in agreement with the results of Han et al. They demonstrated that the DP distance was 0.8 to 6.7 mm in a Chinese population; this distance was 3 to 5 mm in 76.67% of the cases in their study.23

Assessment of the angle formed between the mesiobuccal, distobuccal and palatal (MDP) canal orifices revealed that the mean value of this angle was 106.37°±12.12° (range 80.4° to 144.50°); this value ranged from 90° to 120° in 80% of the cases, which indicates that the distobuccal canal orifice had less of a distal (23.6%) and more of a distopalatal (55.8%) position compared with the first mesiobuccal canal orifice, which was in agreement with the results of previous studies.10,23 Two theories can be suggested for cases in whom this angle was >120°. The first theory relates to the displacement of distobuccal root towards the mesial. The second theory is displacement of the palatal root towards the distal. Both of these occurrences can increase the angle between the orifices. However, further studies are required to further elucidate this topic. Moreover, our study showed that this angle was >120° in 13% of the cases, which indicates that the position of orifice was closer to the line connecting the mesiobuccal and palatal canals. There is a possibility that the load applied from the distal surface causes mesiopalatal movement of distobuccal root during tooth development and eruption due to the thinness of buccal bone, which may complicate canal negotiation during endodontic treatment.23 Alternatively, a load applied from the mesial can cause displacement of palatal root towards the distal. Care must be taken to first negotiate the area distal to the developmental groove connecting the mesiobuccal and palatal canal orifices, which is easily accessible to find the distobuccal canal without additional extension of the access cavity towards the distal. If the canal was not found, the access cavity can be extended distopalatally to find the distobuccal canal orifice. Also, our results showed that this angle was <90° in 7.3% of the cases, which indicates the more buccal position of distobuccal canal orifice or more mesial position of palatal canal orifice, which make it challenging to find the distobuccal canal orifice. Thus, if the canal orifice is not found in distopalatal position or around the line connecting the mesiobuccal and palatal canal orifices, CBCT can be used to evaluate the presence/absence of canal without further extension of the access cavity to avoid missing of a canal. Han et al demonstrated that the MDP angle ranged from 69.4 to 174.7°; this range was 90 to 140° in 92.8% of the cases; it was <90° in 1.83% of the cases.23 Their results were different from ours, which may be attributed to racial differences and different sample size.

Conclusion

The results showed that the position of distobuccal canal orifice was variable in maxillary second molars, which makes it difficult to negotiate this canal. Thus, CBCT can be used to reveal the position of canal orifices especially the distobuccal canal orifice during endodontic treatment.