| Literature DB >> 32367359 |
Jessica L Smith1,2, Alec J Hirsch3,4.
Abstract
Zika virus (ZIKV) is a newly emerged mosquito-borne flavivirus that has been associated with birth defects of babies born to ZIKV-infected mothers. Neutralization activity of serum derived from ZIKV infected and vaccinated individuals is a critical component for characterizing immune response to infection and vaccine efficacy. This protocol describes a modified plaque reduction neutralization 50 (PRNT50) assay that includes an immunostaining step to improve reproducibility and throughput of the assay.Entities:
Keywords: Antibody; Flavivirus; Focus reduction neutralization; Immunostain; Plaque reduction neutralization; Zika virus
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Year: 2020 PMID： 32367359 DOI： 10.1007/978-1-0716-0581-3_6
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745