Rania Alaaeldin1, Maiiada Hassan Nazmy2, Mohamed Abdel-Aziz3, Gamal El-Din A Abuo-Rahma3, Moustafa Fathy4,5. 1. Department of Biochemistry, Faculty of Pharmacy, Deraya University, Minia, Egypt. 2. Department of Biochemistry, Faculty of Pharmacy, Minia University, Minia, Egypt. 3. Department of Medicinal Chemistry, Faculty of Pharmacy, Minia University, Minia, Egypt. 4. Department of Biochemistry, Faculty of Pharmacy, Minia University, Minia, Egypt moustafa_fathyy@yahoo.com. 5. Department of Regenerative Medicine, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan.
Abstract
BACKGROUND/AIM: Ciprofloxacin has been used as an antibiotic in the clinic for decades. Recently, ciprofloxacin and its derivatives have shown promising anti-proliferative and cytotoxic activities against several malignant cells. The aim of this study was to investigate the effect of a new derivative of ciprofloxacin on colorectal cancer (HCT116) and non-small lung carcinoma (A549) cells. MATERIALS AND METHODS: Cell viability was detected by the MTT assay. Flow cytometry was used to examine the cell cycle and apoptosis. Expression of bax, bcl2, p53 and p21 was investigated by qRT-PCR and western blotting. RESULTS: Ciprofloxacin-derivative had an anti-proliferative effect on both cell lines in a concentration-dependent manner and caused cell cycle arrest at the G2/M phase and apoptosis. p53 and Bax proteins were overexpressed, while p21 and bcl2 gene expression was decreased after treatment with the ciprofloxacin derivative. CONCLUSION: This new ciprofloxacin derivative can be potentially used for the treatment of colorectal cancer and non-small lung carcinoma. Copyright
BACKGROUND/AIM: Ciprofloxacin has been used as an antibiotic in the clinic for decades. Recently, ciprofloxacin and its derivatives have shown promising anti-proliferative and cytotoxic activities against several malignant cells. The aim of this study was to investigate the effect of a new derivative of ciprofloxacin on colorectal cancer (HCT116) and non-small lung carcinoma (A549) cells. MATERIALS AND METHODS: Cell viability was detected by the MTT assay. Flow cytometry was used to examine the cell cycle and apoptosis. Expression of bax, bcl2, p53 and p21 was investigated by qRT-PCR and western blotting. RESULTS:Ciprofloxacin-derivative had an anti-proliferative effect on both cell lines in a concentration-dependent manner and caused cell cycle arrest at the G2/M phase and apoptosis. p53 and Bax proteins were overexpressed, while p21 and bcl2 gene expression was decreased after treatment with the ciprofloxacin derivative. CONCLUSION: This new ciprofloxacin derivative can be potentially used for the treatment of colorectal cancer and non-small lung carcinoma. Copyright
Authors: Michael A Fawzy; Sherif A Maher; Mahmoud A El-Rehany; Nermeen N Welson; Nisreen K A Albezrah; Gaber El-Saber Batiha; Moustafa Fathy Journal: Molecules Date: 2022-02-18 Impact factor: 4.411
Authors: Marwa A Eisa; Moustafa Fathy; Gamal El-Din A A Abuo-Rahma; Mohamed Abdel-Aziz; Maiiada Hassan Nazmy Journal: Asian Pac J Cancer Prev Date: 2021-10-01
Authors: Rania Alaaeldin; Heba Ali Hassan; Islam M Abdel-Rahman; Reham H Mohyeldin; Nancy Youssef; Ahmed E Allam; Sayed F Abdelwahab; Qing-Li Zhao; Moustafa Fathy Journal: Curr Issues Mol Biol Date: 2022-07-02 Impact factor: 2.976
Authors: Rania Alaaeldin; Iman A M Abdel-Rahman; Heba Ali Hassan; Nancy Youssef; Ahmed E Allam; Sayed F Abdelwahab; Qing-Li Zhao; Moustafa Fathy Journal: Molecules Date: 2021-12-16 Impact factor: 4.411
Authors: Soad A Mohamed; Mohamed A Abdelgawad; Rania Alaaeldin; Zeinab Fathalla; Hossam Moharram; Raafat M A Abdallah; Islam M Abdel-Rahman; Mohamed Abdel-Aziz; Gamal El-Din A Abuo-Rahma; Mohammed M Ghoneim; Alaa M Hayallah; Mahmoud Elrehany; Hamdy Abdelkader Journal: Pharmaceuticals (Basel) Date: 2021-12-29