Issei Takeuchi1,2, Riko Kawamata1, Kimiko Makino3,2. 1. Faculty of Pharmaceutical Sciences, Tokyo University of Science, Chiba, Japan. 2. Center for Drug Delivery Research, Tokyo University of Science, Chiba, Japan. 3. Faculty of Pharmaceutical Sciences, Tokyo University of Science, Chiba, Japan makino@rs.noda.tus.ac.jp.
Abstract
BACKGROUND/AIM: Oral mucositis, which occurs frequently in the treatment of cancer, is a major problem. In this study, we aimed to develop a rat model of oral mucositis induced by cancer chemotherapy for quantitative measurement. MATERIALS AND METHODS: A model animal of oral mucositis was prepared by injecting an acetic acid aqueous solution into the buccal mucosa of rats to which a 5-FU solution had been previously administered. The doses of 5-FU and acetic acid were examined, and a treatment experiment using Kenalog® was performed. RESULTS: The optimal dose of the 5-FU solution and the optimal concentration of the acetic acid aqueous solution were 40 mg/kg and 25%, respectively. Treatment with Kenalog® confirmed that this model mimics immunocompromised oral mucositis. CONCLUSION: Compared with a mouse model, oral mucositis can be easily observed in this model and provides a large amount of oral mucosal tissue. Copyright
BACKGROUND/AIM: Oral mucositis, which occurs frequently in the treatment of cancer, is a major problem. In this study, we aimed to develop a rat model of oral mucositis induced by cancer chemotherapy for quantitative measurement. MATERIALS AND METHODS: A model animal of oral mucositis was prepared by injecting an acetic acid aqueous solution into the buccal mucosa of rats to which a 5-FU solution had been previously administered. The doses of 5-FU and acetic acid were examined, and a treatment experiment using Kenalog® was performed. RESULTS: The optimal dose of the 5-FU solution and the optimal concentration of the acetic acid aqueous solution were 40 mg/kg and 25%, respectively. Treatment with Kenalog® confirmed that this model mimics immunocompromised oral mucositis. CONCLUSION: Compared with a mouse model, oral mucositis can be easily observed in this model and provides a large amount of oral mucosal tissue. Copyright
Authors: Sung Kyun Yim; Kyoung Min Kim; Chang-Hoon Lee; Eun-Kee Song; Seung Ok Lee; Sang Wook Kim; In Hee Kim; Seong-Hun Kim; Seung Young Seo; Soo Teik Lee Journal: In Vivo Date: 2021 May-Jun Impact factor: 2.155